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Astaxanthin reduces perfluorooctanoic acid cytotoxicity in Saccharomyces cerevisiae.
Toxicology Research ( IF 2.2 ) Pub Date : 2019-11-18 , DOI: 10.1039/c9tx00215d S J Sudharshan 1 , Raghavendra Tirupathi 2 , Madhu Dyavaiah 1
Toxicology Research ( IF 2.2 ) Pub Date : 2019-11-18 , DOI: 10.1039/c9tx00215d S J Sudharshan 1 , Raghavendra Tirupathi 2 , Madhu Dyavaiah 1
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Perfluorooctanoic acid (PFA) has been identified as an environmental contaminant of high concern for human health. In this study, we demonstrated that PFA induces a dose (0 to 1.5 mM) dependent cytotoxicity in S. cerevisiae cells which can be rescued by astaxanthin. The percent sensitivity induced by PFA and the cell protection offered by astaxanthin (30 μM) were demonstrated by CFU counts and spots. The increase in intracellular ROS, superoxide dismutase (SOD), glutathione and lipid peroxidation levels in PFA treated cells suggested that increased oxidative stress resulted in yeast cell death. In contrast, decreased ROS level, increased SOD activity, reduced glutathione and decreased lipid peroxidation by astaxanthin supplementation suggest that the cells are protected from the PFA induced oxidative stress mediated cytotoxicity. Reduced chromatin condensation and nuclear fragmentation in astaxanthin pre-treated cells indicate that astaxanthin rescued the cells from PFA induced apoptosis. Our overall results suggest that PFA induces oxidative stress-mediated cytotoxicity in yeast cells, which were rescued by astaxanthin treatment.
中文翻译：
虾青素可降低酿酒酵母中全氟辛酸的细胞毒性。
全氟辛酸（PFA）已被确定为对人类健康高度关注的环境污染物。在这项研究中，我们证明 PFA 在酿酒酵母细胞中诱导剂量（0 至 1.5 mM）依赖性细胞毒性，虾青素可以挽救这种毒性。PFA 诱导的敏感性百分比和虾青素 (30 μM) 提供的细胞保护通过 CFU 计数和斑点得到证明。PFA 处理的细胞中细胞内 ROS、超氧化物歧化酶 (SOD)、谷胱甘肽和脂质过氧化水平的增加表明氧化应激增加导致酵母细胞死亡。相反，补充虾青素可降低 ROS 水平、增加 SOD 活性、减少谷胱甘肽和减少脂质过氧化，表明细胞受到保护，免受 PFA 诱导的氧化应激介导的细胞毒性。虾青素预处理的细胞中染色质浓缩和核碎裂减少表明虾青素将细胞从 PFA 诱导的细胞凋亡中拯救出来。我们的总体结果表明，PFA 在酵母细胞中诱导氧化应激介导的细胞毒性，而虾青素处理可以挽救这种细胞毒性。
更新日期：2019-11-18
中文翻译：
虾青素可降低酿酒酵母中全氟辛酸的细胞毒性。
全氟辛酸（PFA）已被确定为对人类健康高度关注的环境污染物。在这项研究中，我们证明 PFA 在酿酒酵母细胞中诱导剂量（0 至 1.5 mM）依赖性细胞毒性，虾青素可以挽救这种毒性。PFA 诱导的敏感性百分比和虾青素 (30 μM) 提供的细胞保护通过 CFU 计数和斑点得到证明。PFA 处理的细胞中细胞内 ROS、超氧化物歧化酶 (SOD)、谷胱甘肽和脂质过氧化水平的增加表明氧化应激增加导致酵母细胞死亡。相反，补充虾青素可降低 ROS 水平、增加 SOD 活性、减少谷胱甘肽和减少脂质过氧化，表明细胞受到保护，免受 PFA 诱导的氧化应激介导的细胞毒性。虾青素预处理的细胞中染色质浓缩和核碎裂减少表明虾青素将细胞从 PFA 诱导的细胞凋亡中拯救出来。我们的总体结果表明，PFA 在酵母细胞中诱导氧化应激介导的细胞毒性，而虾青素处理可以挽救这种细胞毒性。
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