The Split Primer Ligation‐triggered 8‐17 DNAzyme Assisted Cascade Rolling Circle Amplification for High Specific Detection of Liver Cancer‐involved mRNAs: TK1 and c‐myc,Electroanalysis

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The Split Primer Ligation‐triggered 8‐17 DNAzyme Assisted Cascade Rolling Circle Amplification for High Specific Detection of Liver Cancer‐involved mRNAs: TK1 and c‐myc
Electroanalysis ( IF 2.7 ) Pub Date : 2019-11-18 , DOI: 10.1002/elan.201900539
Shiyan Dai ₁ , Yuting Zhou ₁ , Peiqing Dai ₁ , Guifang Cheng ₁ , Pingang He ₁ , Yuzhi Fang ₁

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Simultaneous detection of various intracellular biomarkers is promising for early diagnosis and treatment of cancer. Herein, we develop a novel method for high specific and ultrasensitive detection of liver cancer cell‐involved mRNAs: TK1 and c‐myc based on the split primer ligation‐triggered 8‐17 DNAzyme assisted cascade rolling circle amplification. Only two targets exist simultaneously, can trigger the rolling circle amplification to improve the accuracy and sensitivity. Meanwhile, an electrochemical molecular beacon, based on the host‐guest recognition between ferrocene groups and cucurbit urils [7] (CB[7]/Fc‐MB), is used to cause a “turn‐off” electrochemical signal which is decreased by disrupting its hairpin structure. Under the optimal conditions, the detection limit of TK1 and c‐myc mRNA is as low as 0.06 nM. Moreover, this method can be used to detect the TK1 and c‐myc mRNA in HepG2 cells and distinguish between cancer cells and their normal cells, proving that the method has the potential to detect the variation of biomarkers in vivo.

中文翻译：

分裂引物连接触发的8-17 DNAzyme辅助级联滚环扩增技术，用于肝癌相关mRNA的高特异性检测：TK1和c-myc

同时检测各种细胞内生物标志物有望用于癌症的早期诊断和治疗。本文中，我们开发了一种新的方法，可基于分裂引物连接触发的8-17 DNAzyme辅助级联滚环扩增，高特异性和超灵敏地检测肝癌细胞相关mRNA：TK1和c-myc。只有两个目标同时存在，可以触发滚环放大，以提高准确性和灵敏度。同时，基于二茂铁基团和葫芦丝之间的主客体识别[7]（CB [7] / Fc-MB），电化学分子信标被用于引起“关闭”电化学信号，该信号被降低破坏其发夹结构。在最佳条件下，TK1和c-myc mRNA的检出限低至0.06 nM。而且，

更新日期：2019-11-18

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