Purifying Low-Volume Combinatorial Polymer Libraries with Gel Filtration Columns.,Macromolecular Rapid Communications

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Purifying Low-Volume Combinatorial Polymer Libraries with Gel Filtration Columns.
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2019-11-18 , DOI: 10.1002/marc.201900528
Rahul Upadhya ₁ , Mythili J Kanagala ₁ , Adam J Gormley ₁

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Recent advances in oxygen-tolerant controlled/living radical polymer chemistry now enable efficient synthesis of diverse and combinatorial polymer libraries. While library synthesis has been dramatically simplified, equally efficient purification strategies for removal of small-molecule impurities are not yet established in high throughput settings. It is shown that gel filtration columns for chromatography frequently used in the protein science community are well suited for high throughput polymer purification. Using either single-use columns or gel filtration plates, the purification of 32 diverse polymers is demonstrated in a library with >95% removal of small molecule impurities and >85% polymer retention in a single purification step. Doing so replaces the typical procedure of polymer precipitation, which requires solvent optimization for each polymer in a complex library. Overall, this work raises awareness in the polymer science community that gel filtration is amenable to purification of large polymer libraries and can speed up the progress of combinatorial polymer chemistry.

中文翻译：

使用凝胶过滤柱纯化低容量组合聚合物库。

耐氧受控/活性自由基聚合物化学的最新进展现在能够有效合成多种组合聚合物库。虽然文库合成已大大简化，但在高通量设置中尚未建立同样有效的去除小分子杂质的纯化策略。结果表明，蛋白质科学界经常使用的色谱凝胶过滤柱非常适合高通量聚合物纯化。使用一次性柱或凝胶过滤板，在文库中证明了 32 种不同聚合物的纯化，在单个纯化步骤中小分子杂质去除率 > 95%，聚合物保留 > 85%。这样做取代了聚合物沉淀的典型程序，后者需要对复杂库中的每种聚合物进行溶剂优化。总体而言，这项工作提高了聚合物科学界的认识，即凝胶过滤适合纯化大型聚合物库，并且可以加快组合聚合物化学的进展。

更新日期：2019-11-18

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