Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-03 , DOI: 10.1021/acssensors.9b01740 Hua-Ying Chen 1 , Essy Kouadio Fodjo 1, 2 , Lei Jiang 1 , Shuai Chang 1 , Jia-Bin Li 1 , De-Sheng Zhan 1 , Hai-Xin Gu 3 , Da-Wei Li 1
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-03 , DOI: 10.1021/acssensors.9b01740 Hua-Ying Chen 1 , Essy Kouadio Fodjo 1, 2 , Lei Jiang 1 , Shuai Chang 1 , Jia-Bin Li 1 , De-Sheng Zhan 1 , Hai-Xin Gu 3 , Da-Wei Li 1
Affiliation
|
A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO-) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO- but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO-, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10-4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO- in the Raw264.7 cells has been fulfilled by this functional nanosensor, which shows that the SERS strategy will be promising in comprehension of the physiological issues related with NO and ONOO-.
中文翻译：
具有双重反应性的表面增强拉曼散射纳米传感器同时检测细胞内一氧化氮和过氧亚硝酸盐。
探索了一种功能性表面增强拉曼散射（SERS）纳米传感器，该传感器可同时检测活细胞中的一氧化氮（NO）和过氧亚硝酸盐（ONOO-）。通过使用新合成的具有两个反应基团的3,4-二氨基苯基硼酸频哪醇酯（DAPBAP）修饰金纳米颗粒（AuNP），可以制造SERS纳米传感器。在这项工作中实现的同时检测不仅是由于DAPBAP在具有NO和ONOO-的AuNP上的双重反应导致纳米传感器的SERS光谱变化，而且还因为适用于多重检测的窄SERS谱带。由于SERS指纹信息和化学反应特异性的结合，纳米传感器分别对NO和ONOO-具有很高的选择性。此外，纳米传感器具有从0到1的宽线性范围。0×10-4 M，亚微摩尔灵敏度。更重要的是，通过该功能纳米传感器已经实现了Raw264.7细胞中NO和ONOO-的同时监测，这表明SERS策略在理解与NO和ONOO-有关的生理问题方面将很有前途。
更新日期：2019-12-03
中文翻译：
具有双重反应性的表面增强拉曼散射纳米传感器同时检测细胞内一氧化氮和过氧亚硝酸盐。
探索了一种功能性表面增强拉曼散射（SERS）纳米传感器，该传感器可同时检测活细胞中的一氧化氮（NO）和过氧亚硝酸盐（ONOO-）。通过使用新合成的具有两个反应基团的3,4-二氨基苯基硼酸频哪醇酯（DAPBAP）修饰金纳米颗粒（AuNP），可以制造SERS纳米传感器。在这项工作中实现的同时检测不仅是由于DAPBAP在具有NO和ONOO-的AuNP上的双重反应导致纳米传感器的SERS光谱变化，而且还因为适用于多重检测的窄SERS谱带。由于SERS指纹信息和化学反应特异性的结合，纳米传感器分别对NO和ONOO-具有很高的选择性。此外，纳米传感器具有从0到1的宽线性范围。0×10-4 M，亚微摩尔灵敏度。更重要的是，通过该功能纳米传感器已经实现了Raw264.7细胞中NO和ONOO-的同时监测，这表明SERS策略在理解与NO和ONOO-有关的生理问题方面将很有前途。
京公网安备 11010802027423号