Ticks and Tick-Borne Diseases ( IF 3.1 ) Pub Date : 2019-11-17 , DOI: 10.1016/j.ttbdis.2019.101333 Marina V Safonova 1 , Anatoly P Gmyl 2 , Alexander N Lukashev 3 , Anna S Speranskaya 4 , Alexey D Neverov 4 , Gennady G Fedonin 4 , Ekaterina V Pimkina 4 , Alina D Matsvay 4 , Kamil F Khafizov 4 , Galina G Karganova 2 , Lubov I Kozlovskaya 2 , Anna V Valdokhina 4 , Victoria P Bulanenko 4 , Vladimir G Dedkov 5
Kemerovo virus (KEMV) is a member of the Great Island virus genetic group, belonging to the tick-borne arboviruses of the genus Orbivirus within the family Reoviridae.
Nine strains of KEMV, which were isolated from various locations in Russia, were sequenced by high-throughput sequencing to study their intraspecific diversity and the interspecific relationships of viruses within the Great Island genetic group.
For the first time, multiple reassortment within KEMV was reliably demonstrated. Different types of independently emerged alternative reading frames in segment 9 and heterogeneity of the viral population in one of the KEMV strains were found. The hypothesis of the role of an alternative open reading frame (ORF) in segment 9 in KEMV cellular tropism was not confirmed in this study.
中文翻译：
克麦罗沃病毒的遗传多样性和大岛病毒基因组内的系统发育关系。
克麦罗沃病毒（KEMV）是大岛病毒的基因组中的一员，属于属的蜱传虫媒病毒环状病毒家族内呼肠孤病毒。
通过高通量测序对从俄罗斯各地分离出的9种KEMV菌株进行了测序，以研究它们的种内多样性和大岛遗传群内病毒的种间关系。
首次可靠地证明了KEMV内的多种重配。发现了第9部分中不同类型的独立出现的替代阅读框，以及其中一种KEMV菌株中病毒种群的异质性。这项研究中没有证实在第9节中KEMV细胞趋向性的替代开放阅读框（ORF）的作用的假设。
京公网安备 11010802027423号