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Halomonas borealis sp. nov. and Halomonas niordiana sp. nov., two new species isolated from seawater.
Systematic and Applied Microbiology ( IF 3.4 ) Pub Date : 2019-11-17 , DOI: 10.1016/j.syapm.2019.126040
Ana L Diéguez 1 , Sabela Balboa 1 , Jesús L Romalde 1
Affiliation  

Two Gram-negative strains obtained from tank water in a scallop hatchery in Norway, were phenotypically and genotypically characterized in order to clarify their taxonomic position. On the basis of 16S rRNA gene sequence analysis, these isolates, ATF 5.2T and ATF 5.4T, were included in the genus Halomonas, being their closest relatives H. smyrnensis and H. taeanensis, with similarities of 98.9% and 97.7%, respectively. Sequence analysis of the housekeeping genes atpA, ftsZ, gyrA, gyrB, mreB, rpoB, rpoD, rpoE, rpoH, rpoN and rpoS clearly differentiated the isolates from the currently described Halomonas species, and the phylogenetic analysis using concatenated sequences of these genes located them in two robust and independent branches. DNA–DNA hybridization (eDDH) percentage, together with average nucleotide identity (ANI), were calculated using the complete genome sequences of the strains, and demonstrate that the isolates constitute two new species of Halomonas, for which the names of Halomonas borealis sp. nov. and Halomonas niordiana sp. nov. are proposed, with type strains ATF 5.2T (=CECT 9780T = LMG 31367T) and ATF 5.4T (=CECT 9779T = LMG 31227T), respectively.



中文翻译:

北极盐单胞菌 十一月 和Halomonas niordiana sp。11月,从海水中分离出两个新物种。

从表型和基因型上对从挪威扇贝孵化场的储水箱中获得的两种革兰氏阴性菌株进行了表型和基因型鉴定,以阐明它们的分类学地位。根据16S rRNA基因序列分析,这些分离株ATF 5.2 T和ATF 5.4 T被包括在Halomonas属中,它们是近亲H. smyrnensisH. taeanensis,相似度分别为98.9%和97.7%。 。管家基因atp A,fts Z,gyr A,gyr B,mre B,rpo B,rpo D,rpo的序列分析E,rpo H,rpo N和rpo S清楚地将分离物与当前描述的Halomonas物种区分开,并且使用这些基因的串联序列进行的系统发育分析将它们定位在两个强大且独立的分支中。使用菌株的完整基因组序列计算了DNA–DNA杂交(eDDH)百分比以及平均核苷酸同一性(ANI),证明了分离物构成了两个新的Halomonas物种,其名称为Halomonas tubealis sp。十一月 和Halomonas niordiana sp。十一月 建议使用ATF 5.2 T型应变(= CECT 9780 T = LMG 31367 T)和ATF 5.4 T(= CECT 9779 T  = LMG 31227 T)。

更新日期:2019-11-17
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