Abstract In this study we investigated the human red blood cell (RBC) dynamics by means of biospeckle laser analysis. Blood samples from healthy donors were introduced in a 0.8 mm internal diameter capillary tube, and illuminated with a He-Ne laser in order to obtain the biospeckle pattern from both side and forward scattered light. Experiments were carried out for different concentrations of red blood cells in plasma, from 25% to 50%. Biospeckle parameters such as Correlation Coefficient and Inertia Moment, were calculated for different frequency bandwidths in order to assess their sensitivity and versatility. A filter based on the Discrete Wavelet Transform was used to decompose the registered sample activity. A relation between Inertia Moment and the RBCs to plasma volume ratio was observed. The Correlation Index that measures the level of correlation of biospeckle images was defined and analyzed. This work inquires in a technique that is suitable for the development of novel non-invasive optical tools for clinical diagnosis in vascular pathologies.

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生物斑点激光研究毛细管中红细胞动力学的实验方法

摘要 在这项研究中，我们通过生物斑点激光分析研究了人类红细胞 (RBC) 动力学。将健康献血者的血样引入内径为 0.8 mm 的毛细管中，并用 He-Ne 激光照射，以便从两侧和前向散射光获得生物散斑图案。对血浆中不同浓度的红细胞进行了实验，从 25% 到 50%。生物散斑参数，例如相关系数和惯性矩，针对不同的频率带宽进行了计算，以评估它们的灵敏度和通用性。基于离散小波变换的滤波器用于分解注册的样本活动。观察到惯性矩与红细胞与血浆体积比之间的关系。定义并分析了测量生物斑点图像相关性水平的相关性指数。这项工作探索了一种适合开发用于血管病理学临床诊断的新型非侵入性光学工具的技术。