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Simultaneous determination of artemisinin and its analogues and flavonoids in Artemisia Annua crude extracts with the use of NMR Spectroscopy
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2020-01-07 , DOI: 10.1002/mrc.4971 Vassiliki G Kontogianni 1 , Alexandra Primikyri 1 , Marianna Sakka 1 , Ioannis P Gerothanassis 1
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2020-01-07 , DOI: 10.1002/mrc.4971 Vassiliki G Kontogianni 1 , Alexandra Primikyri 1 , Marianna Sakka 1 , Ioannis P Gerothanassis 1
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Artemisia annua is a promising and potent antimalarial herbal drug. This activity has been ascribed to its component artemisinin, a sesquiterpene lactone. The ability to determine artemisinin and its known analogs in plant extracts is an especially difficult task because the compounds are present in low concentrations, are thermolabile, and lack ultraviolet or fluorescent chromophores. We report herein a facile and rapid 1‐D 1H, 1‐D total correlation spectroscopy, 2‐D 1H–13C heteronuclear single quantum coherence, and 1H–13C heteronuclear multiple bond correlation nuclear magnetic resonance techniques for the simultaneous identification and quantification of artemisinin and five of its analogs along with five flavonoids, an aromatic ketone, and camphor (in total, 13 compounds) in crude diethyl ether A. annua extract without the need of laborious isolation of the individual analytes. The above method was validated in terms of precision, linearity, and limit of detection. The analytical results were found to be in excellent agreement with those obtained with the use of the time consuming high‐performance liquid chromatography with diode‐array detection and liquid chromatography with tandem mass spectrometry for the compounds that standards were available.
中文翻译：
使用核磁共振波谱法同时测定青蒿粗提物中的青蒿素及其类似物和黄酮类化合物
青蒿是一种有前途且有效的抗疟草药。这种活性归因于其成分青蒿素，一种倍半萜内酯。确定植物提取物中的青蒿素及其已知类似物的能力是一项特别困难的任务，因为这些化合物的浓度很低，不耐热，并且没有紫外线或荧光发色团。我们在此报告了一种简便快速的 1-D 1H、1-D 全相关光谱、2-D 1H-13C 异核单量子相干和 1H-13C 异核多键相关核磁共振技术，用于同时鉴定和定量青蒿素及其五种类似物以及五种黄酮类化合物、一种芳香酮和樟脑（总共 13 种化合物）在粗乙醚 A 中。无需费力分离单个分析物即可提取青蒿提取物。上述方法在精密度、线性和检测限方面得到验证。发现分析结果与使用耗时的高效液相色谱与二极管阵列检测和液相色谱与串联质谱法对标准可用的化合物获得的结果非常一致。
更新日期：2020-01-07
中文翻译：
使用核磁共振波谱法同时测定青蒿粗提物中的青蒿素及其类似物和黄酮类化合物
青蒿是一种有前途且有效的抗疟草药。这种活性归因于其成分青蒿素，一种倍半萜内酯。确定植物提取物中的青蒿素及其已知类似物的能力是一项特别困难的任务，因为这些化合物的浓度很低，不耐热，并且没有紫外线或荧光发色团。我们在此报告了一种简便快速的 1-D 1H、1-D 全相关光谱、2-D 1H-13C 异核单量子相干和 1H-13C 异核多键相关核磁共振技术，用于同时鉴定和定量青蒿素及其五种类似物以及五种黄酮类化合物、一种芳香酮和樟脑（总共 13 种化合物）在粗乙醚 A 中。无需费力分离单个分析物即可提取青蒿提取物。上述方法在精密度、线性和检测限方面得到验证。发现分析结果与使用耗时的高效液相色谱与二极管阵列检测和液相色谱与串联质谱法对标准可用的化合物获得的结果非常一致。
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