In this study, polylactic acid (PLA)-based composite scaffolds with calcium carbonate (CaCO3 ) and beta-tricalcium phosphate (β-TCP) were obtained by 3D printing. These structures were evaluated as potential 3D structures for bone tissue regeneration. Morphological, mechanical, and biological tests were carried out in order to compare the effect of each additive (added in a concentration of 5% w/w) and the combination of both (2.5% w/w of each one), on the PLA matrix. The scaffolds manufactured had a mean pore size between 400-425 μm and a porosity value in the range of 50-60%. According to the results, both additives promoted an increase of the porosity, hydrophilicity, and surface roughness of the scaffolds, leading to a significant improvement of the metabolic activity of human osteoblastic osteosarcoma cells. The best results in terms of cell attachment after 7 days were obtained for the samples containing CaCO3 and β-TCP particles due to the synergistic effect of both additives, which results in an increase in osteoconductivity and in a microporosity that favours cell adhesion. These scaffolds (PLA:CaCO3 :β-TCP 95:2.5:2.5) have suitable properties to be further evaluated for bone tissue engineering applications.

中文翻译：

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碳酸钙和β-磷酸三钙作为3D打印支架与聚乳酸基质的添加剂的比较。

在这项研究中，通过3D打印获得了具有碳酸钙（CaCO3）和β-磷酸三钙（β-TCP）的基于聚乳酸（PLA）的复合支架。这些结构被评估为骨组织再生的潜在3D结构。为了比较每种添加剂（添加浓度为5％w / w）和两者的组合（每种浓度为2.5％w / w），进行了形态，机械和生物学测试。矩阵。所制造的支架的平均孔径在400-425μm之间，并且孔隙率值在50-60％的范围内。根据结果​​，两种添加剂均促进了支架的孔隙率，亲水性和表面粗糙度的增加，从而导致人成骨骨肉瘤细胞的代谢活性显着改善。由于两种添加剂的协同作用，对于含有CaCO3和β-TCP颗粒的样品，在7天后的细胞附着方面获得了最佳结果，这导致了骨传导性的提高和有利于细胞粘附的微孔性。这些支架（PLA：CaCO3：β-TCP95：2.5：2.5）具有合适的特性，需要进一步评估以用于骨组织工程应用。