Three different cDNA sequences, designated OepFAD2-3, OepFAD2-4 and OepFAD2-5, encoding three microsomal oleate desaturases (FAD2) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis and functional expression in yeast of the corresponding cDNAs confirm that they encode microsomal oleate desaturases. Gene expression and lipid analysis indicate that these three genes are not involved in the linoleic acid present in seed lipids, while OeFAD2-5, together with OeFAD2-2, contributes mostly to the linoleic acid present in the mesocarp and, therefore, in the olive oil. Our results have also shown that olive FAD2-3, FAD2-4 and FAD2-5 gene expression is not only spatially and temporally regulated in olive fruit, but also is cultivar-dependent, as well as regulated by water regime, temperature, light and wounding. All these data suggest specialized physiological roles for the olive FAD2 gene family members with respect to both aspects of the biosynthesis of the linoleic acid, either present in storage lipids that constitute the olive oil or being part of membrane lipids, which are involved in the response to abiotic stresses, and highlight the differences on FAD2 gene regulation between oilseeds and oil fruits.

中文翻译：

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橄榄FAD2基因家族成员与水果发育和非生物胁迫响应相关的特殊功能。

已从橄榄中分离出三个不同的cDNA序列，分别命名为OepFAD2-3，OepFAD2-4和OepFAD2-5，它们编码三个微粒体油酸去饱和酶（FAD2）（Olea europaea cv。Picual）。序列分析和相应的cDNA在酵母中的功能性表达证实它们编码了微粒体油酸去饱和酶。基因表达和脂质分析表明，这三个基因不参与种子脂质中存在的亚油酸，而OeFAD2-5和OeFAD2-2共同作用于中果皮和橄榄中的亚油酸。油。我们的研究结果还表明，橄榄FAD2-3，FAD2-4和FAD2-5基因的表达不仅在空间和时间上都在橄榄果中调控，而且还与品种有关，并且受水分，温度，光照和水分的调控。伤人。