Dermis-fat composite tissues have been widely used in plastic and reconstructive surgery and were previously constructed using hydrogel-type scaffolds. The constructs can be used for in vitro cosmetic and pharmaceutical testing but are not mechanically strong enough for in vivo applications. In this study, we used heterogeneous (porcine) acellular dermal matrix (PADM) as dermal layer scaffold. PADM was pretreated with the laser micropore technique and then precultured with rat adipose-derived stem cells (rADSCs) in vitro. rADSCs proliferated well on pretreated/unpretreated PADM, showing increased expression of genes associated with inflammatory regulation, proangiogenesis, and stemness, indicating that pretreated/unpretreated PADM both provide a beneficial microenvironment for rADSCs to exert their paracrine function. After in vitro processing, the rADSCs-polyporous PADM and PADM without pretreatments were implanted into the back of rats respectively, followed by adipose tissue transplantation. After implantation, the inflammation induced by pretreated PADM was significantly attenuated and localized compared to the unpretreated group. Moreover, the vascularization was faster, and more adipose tissue was formed in the pretreated group. Sound dermis-fat composite tissue was constructed with sufficient strength, which can potentially be used for actual repair application.

中文翻译：

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体内真皮脂肪复合材料的构建：通过体外预处理优化异质脱细胞真皮基质。

真皮脂肪复合组织已广泛用于整形和重建手术中，并且先前使用水凝胶型支架构建。该构建体可用于体外美容和药物测试，但机械强度不足以用于体内应用。在这项研究中，我们使用异质（猪）脱细胞真皮基质（PADM）作为真皮层支架。用激光微孔技术预处理PADM，然后在体外用大鼠脂肪干细胞（rADSC）进行预培养。rADSCs在预处理/未预处理的PADM上增殖良好，显示与炎症调节，促血管生成和干性相关的基因表达增加，表明预处理/未预处理的PADM都为rADSCs发挥旁分泌功能提供了有利的微环境。经过体外处理 将未经预处理的rADSCs-多孔PADM和PADM分别植入大鼠背部，然后进行脂肪组织移植。植入后，与未预处理的组相比，经预处理的PADM诱导的炎症明显减轻和局部化。此外，在预处理组中，血管形成更快，并且形成了更多的脂肪组织。合理的真皮脂肪复合组织构造出足够的强度，可潜在地用于实际修复应用。预处理组的血管形成更快，并且形成更多的脂肪组织。合理的真皮脂肪复合组织构造出足够的强度，可潜在地用于实际修复应用。预处理组的血管形成更快，并且形成了更多的脂肪组织。合理的真皮脂肪复合组织构造出足够的强度，可潜在地用于实际修复应用。