Hydrogen peroxide (H₂O₂) is the main product of enzymatic reactions and plays an important role in biological processes. The detection of H₂O₂ inside organisms or cells is critical. Here, we report a nickel–iron Prussian blue analogue nanocube doped with molybdenum disulfide and Au nanoparticles (Au@PBA(Ni–Fe):MoS₂) as an electrochemical sensing material for the stable detection of H₂O₂ in neutral solutions for a long time. First, the Prussian blue analogue (PBA(Ni–Fe)) is synthesized by a simple charge-assembly technology, and then etched into PBA(Ni–Fe):MoS₂ hollow nanocubes by a high-temperature hydrothermal reaction. Finally, Au nanoparticles are reduced inside the PBA(Ni–Fe):MoS₂in situ to generate Au@PBA(Ni–Fe):MoS₂ nanocubes. Ni-doping enhances the nanocube's stability in neutral solutions; as a result, the sensor can maintain a stable current response towards H₂O₂ reduction for more than 1 h. The sensing material can meet the needs of a long-time test. The introduction of Au enhances the electron transfer efficiency, which endows the sensor with good reduction ability for H₂O₂ at 0 V over a wide linear range (0.5–200 μM and 210–3000 μM) and with a low detection limit (0.23 μM (S/N = 3)), which fulfills the requirements for the detection of H₂O₂ in a biological system. The sensor can sense H₂O₂ released from cells stimulated by ascorbic acid. Au@PBA(Ni–Fe):MoS₂ provides good guidance for the future development of efficient biosensors to be applied in cell biology.

中文翻译：

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基于Au @ PBA（Ni-Fe）：MoS2纳米立方体的非酶电化学生物传感器，用于稳定，灵敏地检测从活细胞释放的过氧化氢。

过氧化氢（H ₂ O ₂）是酶促反应的主要产物，在生物过程中起着重要作用。检测生物或细胞内部的H ₂ O ₂至关重要。在这里，我们报告了一种镍铁普鲁士蓝类似物纳米立方体，其中掺杂有二硫化钼和Au纳米颗粒（Au @ PBA（Ni–Fe）：MoS ₂）作为电化学传感材料，用于稳定检测中性溶液中的H ₂ O ₂很长时间。首先，通过简单的电荷组装技术合成普鲁士蓝类似物（PBA（Ni–Fe）），然后将其蚀刻成PBA（Ni–Fe）：MoS ₂中空纳米立方体通过高温水热反应。最后，Au纳米颗粒在PBA（Ni-Fe）：MoS _2内部原位还原，生成Au @ PBA（Ni-Fe）：MoS ₂纳米立方体。镍掺杂增强了纳米立方体在中性溶液中的稳定性；结果，该传感器可以保持对H ₂ O ₂还原的稳定电流响应超过1小时。传感材料可以满足长期测试的需求。Au的引入提高了电子转移效率，这赋予了传感器对H ₂ O _2的良好还原能力。在0 V的线性范围（0.5–200μM和210–3000μM）和低检测限（0.23μM（S / N = 3））范围内，满足了H ₂ O ₂的检测要求。生物系统。该传感器可以感应从抗坏血酸刺激的细胞释放的H ₂ O ₂。Au @ PBA（Ni-Fe）：MoS ₂为细胞生物学中高效生物传感器的未来发展提供了良好的指导。