Metal-organic frameworks (MOFs) are an important class of inorganic-organic hybrid crystals with applications in various fields. In last ten years, MOFs-based fluorescence sensing has gained much attention. MOFs can exhibit luminescence by metal nodes, ligands and the inserted or absorbed guests, which offer an excellent fluorescence response in analyzing. However, MOFs-based monochromatic fluorescence probes are limited by concentration, the effect of ambient and excitation light intensity, resulting in low detection accuracy. To overcome this defect, MOFs-based ratiometric fluorescence (RF) sensors have been proposed and rapidly developed. This review focuses on the design tactics of MOFs-based RF sensing and describes the detection mechanism of different RF sensors. Based on different strategies of synthesis, the MOFs-based RF sensors are categorized into three classes: MOFs’ intrinsic dual-emission, single-emissive MOFs with a chromophore and non-emissive MOFs with two chromophores. In each categorization, the design approaches and relative merits were discussed separately. Finally, the applications of MOFs-based RF sensors are summarized and prospected.

金属有机骨架（MOF）是一类重要的无机有机杂化晶体，在各种领域中都有应用。在过去的十年中，基于MOF的荧光传感已引起了广泛关注。MOF可以通过金属节点，配体以及插入或吸收的客体显示出发光，从而在分析中提供出色的荧光响应。但是，基于MOF的单色荧光探针受浓度，环境光和激发光强度的影响，从而导致检测精度低。为了克服该缺陷，已经提出并迅速开发了基于MOF的比例荧光（RF）传感器。这篇综述着重于基于MOF的RF感应的设计策略，并描述了不同RF传感器的检测机制。根据不同的合成策略，基于MOF的RF传感器可分为三类：MOF的固有双发射，带发色团的单发射MOF和带两个发色团的非发射MOF。在每种分类中，都分别讨论了设计方法和相对优点。最后，对基于MOF的RF传感器的应用进行了总结和展望。