Cypovirus is an insect virus that is encapsulated in stable cubic protein crystals composed of polyhedrin protein produced in virus-infected cells. Molecular technology developed over the last decade is now able to immobilise proteins of interest on polyhedrin crystals. Modified polyhedrin crystals can be used in cell cultures for implantation in animals and vaccines, among other applications. However, this technique does not work for some proteins. Here, we developed and tested an alternative approach for immobilising foreign proteins in polyhedrin crystals using a linker method; diverse proteins, such as fluorescent proteins, enzymes, antibodies, and streptavidin were successfully contained. The immobilised antibodies retained their binding activity on filter paper, implying their potential for new immunochromatography applications. Moreover, this immobilisation method allows enzymes to be collected from one reaction reagent and transferred to another reagent. These results demonstrate the potential of this immobilisation method and the likelihood of expanding the applications of polyhedrin crystals using this approach.

中文翻译：

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将多种蛋白质固定在立方微蛋白质多面体晶体上的新方法。

杯状病毒是一种昆虫病毒，被封装在稳定的立方蛋白质晶体中，该晶体由病毒感染细胞中产生的多面体蛋白组成。过去十年发展起来的分子技术现在能够将目标蛋白质固定在多面体晶体上。修饰的多面体晶体可用于细胞培养中，以植入动物和疫苗中，以及其他应用。但是，该技术不适用于某些蛋白质。在这里，我们开发并测试了一种使用连接子方法将异源蛋白质固定在多面体晶体中的替代方法；成功地包含了多种蛋白质，例如荧光蛋白，酶，抗体和链霉亲和素。固定的抗体在滤纸上保留了其结合活性，这暗示了它们在新的免疫色谱应用中的潜力。而且，这种固定方法允许从一种反应试剂中收集酶并将其转移到另一种试剂中。这些结果证明了这种固定方法的潜力以及使用这种方法扩展多面体晶体应用的可能性。