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Molecular characterization and expression analysis of fibrinogen related protein (FREP) genes of Manila clam (Ruditapes philippinarum) after lipopolysaccharides challenge.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-11-14 , DOI: 10.1016/j.cbpc.2019.108672 Dongdong Li 1 , Hongtao Nie 1 , Kunyin Jiang 1 , Ning Li 1 , Zhongming Huo 1 , Xiwu Yan 1
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-11-14 , DOI: 10.1016/j.cbpc.2019.108672 Dongdong Li 1 , Hongtao Nie 1 , Kunyin Jiang 1 , Ning Li 1 , Zhongming Huo 1 , Xiwu Yan 1
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Ruditapes philippinarum has high economic value and is distributed all over the world. Fibrinogen associated protein (FREP) is a type of pattern recognition receptor, participates in the innate immune response to eliminate pathogens after the invasion of pathogenic microorganisms. In this study, three FREP genes (FREP-1, FREP-2, and FREP-3) were identified and characterized from R. philippinarum. The protein sequence of FREPs were highly conserved with those homologous in vertebrates, and FBG domain possessed significantly high structural conservation in polypeptide binding site and Ca2+ binding site. The tissues expression analysis of FREPs in three shell color strains and two population of R. philippinarum were examined, with the highest expression level in gill and hepatopancreas. Besides, FREP genes were demonstrated to be induced by lipopolysaccharides injection, the significantly changes were observed after LPS injected. Our results suggest the involvement of FREPs in response to LPS injection, and it might exert a significant function on the innate immune defense of the Manila clam.
中文翻译：
脂多糖激发后马尼拉蛤（Ruditapes philippinarum）纤维蛋白原相关蛋白（FREP）基因的分子表征和表达分析。
Ruditapes philippinarum具有很高的经济价值，并分布于世界各地。纤维蛋白原相关蛋白（FREP）是一种模式识别受体，在病原微生物入侵后参与先天免疫反应以消除病原体。在这项研究中，鉴定了三个FREP基因（FREP-1，FREP-2和FREP-3），并从菲律宾蛤仔中鉴定出了特征。FREPs的蛋白质序列与脊椎动物中的FREPs高度保守，并且FBG结构域在多肽结合位点和Ca2 +结合位点具有显着高的结构保守性。进行了FREPs在三个壳色菌株和两个菲律宾蛤仔中的组织表达分析，其中with和肝胰腺中的表达水平最高。除了，FREP基因被证明是由脂多糖注射诱导的，注射LPS后观察到显着变化。我们的结果表明FREPs参与了LPS注射，并且可能对马尼拉蛤的先天免疫防御起重要作用。
更新日期：2019-11-14
中文翻译：
脂多糖激发后马尼拉蛤（Ruditapes philippinarum）纤维蛋白原相关蛋白（FREP）基因的分子表征和表达分析。
Ruditapes philippinarum具有很高的经济价值，并分布于世界各地。纤维蛋白原相关蛋白（FREP）是一种模式识别受体，在病原微生物入侵后参与先天免疫反应以消除病原体。在这项研究中，鉴定了三个FREP基因（FREP-1，FREP-2和FREP-3），并从菲律宾蛤仔中鉴定出了特征。FREPs的蛋白质序列与脊椎动物中的FREPs高度保守，并且FBG结构域在多肽结合位点和Ca2 +结合位点具有显着高的结构保守性。进行了FREPs在三个壳色菌株和两个菲律宾蛤仔中的组织表达分析，其中with和肝胰腺中的表达水平最高。除了，FREP基因被证明是由脂多糖注射诱导的，注射LPS后观察到显着变化。我们的结果表明FREPs参与了LPS注射，并且可能对马尼拉蛤的先天免疫防御起重要作用。
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