Small Molecule as Fluorescent Probes for Monitoring Intracellular Enzymatic Transformations.,Chemical Reviews

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Small Molecule as Fluorescent Probes for Monitoring Intracellular Enzymatic Transformations.
Chemical Reviews ( IF 51.4 ) Pub Date : 2019-11-14 , DOI: 10.1021/acs.chemrev.9b00379
Harwinder Singh ₁ , Karishma Tiwari ₁ , Rajeshwari Tiwari ₁ , Sumit Kumar Pramanik ₁ , Amitava Das ₁

Affiliation

All cellular processes are the results of synchronized actions of several intracellular biochemical pathways. Recent emphasis is to visualize such pathways using appropriate small molecular reagents, dye-labeled proteins, and genetically encoded fluorescent biosensors that produce a luminescence ON response either on selective binding or on reacting with an analyte that is produced through a specific biochemical/enzymatic transformation. Studying such enzymatic processes by probing the fluorescence response as the read-out signal is expected to provide important insights into crucial biochemical transformations induced by an enzyme in its native form. Many of such studies are extended for monitoring enzymatic transformations under in vitro or in vivo condition. A few of the recent reports reveal that such molecular probes are even capable of quantifying abnormal levels of enzymes in real-time and is linked to the key area of clinical diagnostics and chemical biology. A synchronized analysis of all such reports helps in developing a rationale for designing purpose-built molecular probes or chemodosimeters as well as newer reagents for studying crucial enzymatic process or quantification of the respective enzyme. In this review, an attempt will be there to highlight several recent bioimaging reagents and studies that have provided insights into crucial biochemical or enzymatic transformations.

中文翻译：

小分子作为监测细胞内酶促转化的荧光探针。

所有细胞过程都是几种细胞内生化途径同步作用的结果。最近的重点是使用适当的小分子试剂，染料标记的蛋白质和遗传编码的荧光生物传感器来可视化此类途径，这些传感器在选择性结合或与通过特定生化/酶促转化产生的分析物反应时产生发光ON响应。通过探查作为读出信号的荧光响应来研究这种酶促过程，有望为由天然形式的酶诱导的关键生化转化提供重要的见识。许多这样的研究扩展到在体外或体内条件下监测酶促转化。最近的一些报道表明，这种分子探针甚至能够实时定量酶的异常水平，并且与临床诊断和化学生物学的关键领域有关。对所有此类报告的同步分析有助于开发设计专用分子探针或化学计量计以及用于研究关键酶促过程或相应酶定量的新型试剂的原理。在这篇综述中，将尝试着重介绍几种最近的生物成像试剂和研究，这些研究和研究为重要的生化或酶促转化提供了见识。对所有此类报告的同步分析有助于开发设计专用分子探针或化学计量计以及用于研究关键酶促过程或相应酶定量的新型试剂的原理。在这篇综述中，将尝试着重介绍几种最近的生物成像试剂和研究，这些研究和研究为重要的生化或酶促转化提供了见识。对所有此类报告的同步分析有助于开发设计专用分子探针或化学计量计以及用于研究关键酶促过程或相应酶定量的新型试剂的原理。在这篇综述中，将尝试着重介绍几种最近的生物成像试剂和研究，这些研究和研究为重要的生化或酶促转化提供了见识。

更新日期：2019-11-14

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