Lead (Pb), which widely recognized as a nonessential heavy metal and a major environmental contamination, is a growing threat to the ecosystem and human body. In the present study, Malus micromalus Makino cv. 'Dong Hong' phenolic extract (MMPE) has been used to antagonise Pb-induced erythrocyte injury, hepatic and renal dysfunction in mice. Six-week-old male Kunming mice were gavaged with PbCl2 (20 mg/kg mouse/day) and/or MMPE (100 mg/kg mouse/day) by gavage administration for 10 days. We evaluated erythrocyte fragility, relative organ mass, biochemical parameters and histopathological changes to evaluate the protection effect of MMPE on the injury of liver and kidney in Pb-treated mice. MMPE significantly inhibited the increase of protein kinase C-α, B-cell lymphoma-2-associated X, cytochrome C and Caspase-3 protein levels and decreased calreticulin protein expression level in Pb-exposed mice. MMPE supplementation could maintain the integrity of erythrocyte membranes and ameliorate the endoplasmic reticulum stress in Pb-treated mice. It suggested MMPE as a natural nutritional supplement to alleviate Pb-induced hazardous effects in Pb-exposed humans.

中文翻译：

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苹果属苹果树（Malino micromalus Makino）酚类提取物可通过调节PKC-α信号通路并减轻铅（II）暴露小鼠的内质网应激来维持肝肾功能。

铅（Pb）被广泛认为是不必要的重金属和主要的环境污染，对生态系统和人体的威胁正在日益增加。在目前的研究中，苹果属苹果属苹果属。“东红”酚提取物（MMPE）已被用于拮抗铅诱导的小鼠红细胞损伤，肝肾功能障碍。通过管饲法给六周大的雄性昆明小鼠灌胃PbCl2（20 mg / kg小鼠/天）和/或MMPE（100 mg / kg小鼠/天）。我们评估了红细胞脆性，相对器官质量，生化参数和组织病理学变化，以评估MMPE对Pb治疗的小鼠肝脏和肾脏损伤的保护作用。MMPE显着抑制蛋白激酶C-α，B细胞淋巴瘤2相关X的增加，Pb暴露的小鼠体内细胞色素C和Caspase-3蛋白水平和钙网蛋白蛋白表达水平降低。MMPE补充剂可以维持Pb治疗的小鼠的红细胞膜的完整性并减轻内质网的应激。它建议MMPE作为天然营养补充剂，以减轻Pb暴露于人体的铅引起的有害影响。