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Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-SBP-1 as a target for its management.
International Journal for Parasitology ( IF 3.7 ) Pub Date : 2019-11-14 , DOI: 10.1016/j.ijpara.2019.09.002
Tagginahalli N Shivakumara 1 , Vishal Singh Somvanshi 1 , Victor Phani 1 , Sonam Chaudhary 1 , Alkesh Hada 1 , Roli Budhwar 2 , Rohit Nandan Shukla 2 , Uma Rao 1
Affiliation  

Meloidogyne incognita is a polyphagous plant-parasitic nematode that causes considerable yield loss in agricultural and horticultural crops. The management options available for M. incognita are extremely limited. Here we identified and characterised a M. incognita homolog of Caenorhabditis elegans sterol-binding protein (Mi-SBP-1), a transcriptional regulator of several lipogenesis pathway genes, and used RNA interference-mediated gene silencing to establish its utility as a target for the management of M. incognita. Mi-sbp-1 is predicted to be a helix-loop-helix domain containing DNA binding transcription factor, and is present in the M. incognita genome in three copies. The RNA-Seq analysis of Mi-sbp-1 silenced second stage juveniles confirmed the key role of this gene in lipogenesis regulation in M. incognita. In vitro and host-induced gene silencing of Mi-sbp-1 in M. incognita second stage juveniles resulted in loss of nematodes' ability to utilise the stored fat reserves, slower nematode development, and reduced parasitism on adzuki bean and tobacco plants. The multiplication factor for the Mi-sbp-1 silenced nematodes on adzuki bean plants was reduced by 51% compared with the control nematodes in which Mi-sbp-1 was not silenced. Transgenic expression of the double-stranded RNA construct of the Mi-sbp-1 gene in tobacco plants caused 40-45% reduction in M. incognita multiplication, 30-43.8% reduction in the number of egg masses, and 33-54% reduction in the number of eggs per egg mass compared with the wild type control plants. Our results confirm that Mi-sbp-1 is a key regulator of lipogenesis in M. incognita and suggest that it can be used as an effective target for its management. The findings of this study can be extended to develop methods to manage other economically important parasitic nematodes.

中文翻译:


南方根结线虫(线虫纲:根结线虫科)甾醇结合蛋白 Mi-SBP-1 作为其管理目标。



南方根结线虫是一种多食性植物寄生线虫,会导致农业和园艺作物严重减产。可用于南方根结线虫的管理选择极其有限。在这里,我们鉴定并鉴定了秀丽隐杆线虫甾醇结合蛋白(Mi-SBP-1)的同源物,该蛋白是几种脂肪生成途径基因的转录调节因子,并使用 RNA 干扰介导的基因沉默来确定其作为南方根结线虫的管理。 Mi-sbp-1 被预测为含有 DNA 结合转录因子的螺旋-环-螺旋结构域,并且以三个拷贝存在于南方根结线虫基因组中。 Mi-sbp-1 沉默的第二阶段幼虫的 RNA-Seq 分析证实了该基因在南方根结线虫脂肪生成调节中的关键作用。南方根结线虫第二阶段幼虫中 Mi-sbp-1 的体外和宿主诱导基因沉默导致线虫丧失利用储存的脂肪储备的能力,减慢线虫发育,并减少对红小豆和烟草植物的寄生。与未沉默Mi-sbp-1的对照线虫相比,Mi-sbp-1沉默的线虫在小豆植物上的增殖因子降低了51%。 Mi-sbp-1 基因的双链 RNA 构建体在烟草植物中的转基因表达导致南方根结线虫繁殖减少 40-45%,卵块数量减少 30-43.8%,并减少 33-54%与野生型对照植物相比,每个卵块的卵数。我们的结果证实 Mi-sbp-1 是南方根结线虫脂肪生成的关键调节因子,并表明它可以作为其管理的有效靶标。 这项研究的结果可以扩展到开发管理其他经济上重要的寄生线虫的方法。
更新日期:2019-11-14
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