Odd‐chain fatty acids (OCFA) are widely used in pharmaceutical and food industries, as well as the chemical industry. In this study, oil rich in OCFA was produced by Rhodococcus opacus PD630, and 1‐propanol in combination with glucose was used as the carbon and energy source. Oil was extracted by the subcritical extraction system. Gas chromatography (GC), ultra‐performance liquid chromatography‐mass spectrometry (UPLC‐MS), and differential scanning calorimetry (DSC) were used to evaluate the fatty acids composition, triacylglycerol (TAG) molecular species, and thermal properties, respectively. It was found that the content of OCFA reached 68.15%, where pentadecanoic acid (7.74%), heptadecanoic acid (18.20%), and heptadecenoic acid (42.71%) were the dominant OCFA. The most abundant TAG were HePaHa (15.82%), HePHa (11.42%), HePaHe (9.68%), HeHaHa (9.62%), HePaO (8.26%), and HePPa (8.00%). In terms of thermal profiles, recrystallization was detected in the oil rich in OCFA. Above all, these findings greatly extend the utilization of the microbial oil rich in OCFA, and they may have a significant impact on the future development of the microbial oil industry.

中文翻译：

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不透明红球菌PD630的富含奇链脂肪酸的油的制备与表征

奇数脂肪酸（OCFA）广泛用于制药和食品工业以及化学工业。在这项研究中，不透明红球菌生产了富含OCFA的油PD630和1-丙醇与葡萄糖结合用作碳和能源。通过亚临界萃取系统萃取了油。气相色谱（GC），超高效液相色谱质谱（UPLC-MS）和差示扫描量热法（DSC）分别用于评估脂肪酸组成，三酰基甘油（TAG）分子种类和热性质。发现OCFA的含量达到68.15％，其中十五烷酸（7.74％），十七烷酸（18.20％）和十七烯酸（42.71％）是主要的OCFA。最丰富的TAG是HePaHa（15.82％），HePHa（11.42％），HePaHe（9.68％），HeHaHa（9.62％），HePaO（8.26％）和HePPa（8.00％）。就热曲线而言，在富含OCFA的油中检测到重结晶。首先，