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Molecule-Membrane Interactions in Biological Cells Studied with Second Harmonic Light Scattering.
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2019-12-10 , DOI: 10.1002/asia.201901406
Michael J Wilhelm 1 , Hai-Lung Dai 1
Affiliation  

The nonlinear optical phenomenon second harmonic light scattering (SHS) can be used for detecting molecules at the membrane surfaces of living biological cells. Over the last decade, SHS has been developed for quantitatively monitoring the adsorption and transport of small and medium size molecules (both neutral and ionic) across membranes in living cells. SHS can be operated with both time and spatial resolution and is even capable of isolating molecule-membrane interactions at specific membrane surfaces in multi-membrane cells, such as bacteria. In this review, we discuss select examples from our lab employing time-resolved SHS to study real-time molecular interactions at the plasma membranes of biological cells. We first demonstrate the utility of this method for determining the transport rates at each membrane/interface in a Gram-negative bacterial cell. Next, we show how SHS can be used to characterize the molecular mechanism of the century old Gram stain protocol for classifying bacteria. Additionally, we examine how membrane structures and molecular charge and polarity affect adsorption and transport, as well as how antimicrobial compounds alter bacteria membrane permeability. Finally, we discuss adaptation of SHS as an imaging modality to quantify molecular adsorption and transport in sub-cellular regions of individual living cells.

中文翻译:

用二次谐波光散射研究生物细胞中的分子-膜相互作用。

非线性光学现象二次谐波光散射(SHS)可用于检测生物细胞膜表面的分子。在过去的十年中,已经开发出SHS来定量监测小分子和中型分子(中性和离子性)在活细胞中跨膜的吸附和运输。SHS可以在时间和空间分辨率下运行,甚至能够隔离多膜细胞(例如细菌)中特定膜表面的分子-膜相互作用。在这篇综述中,我们讨论了采用时间分辨SHS的实验室中精选的实例,以研究生物细胞质膜上的实时分子相互作用。我们首先证明该方法用于确定革兰氏阴性细菌细胞中每个膜/界面的转运速率的实用性。接下来,我们展示如何使用SHS来表征具有百年历史的革兰氏染色方案对细菌进行分类的分子机制。此外,我们研究了膜结构以及分子电荷和极性如何影响吸附和转运,以及抗菌化合物如何改变细菌的膜通透性。最后,我们讨论了SHS作为一种成像方式的适应性,以量化单个活细胞亚细胞区域中的分子吸附和转运。我们研究了膜结构,分子电荷和极性如何影响吸附和运输,以及抗菌化合物如何改变细菌的膜通透性。最后,我们讨论了SHS作为一种成像方式的适应性,以量化单个活细胞亚细胞区域中的分子吸附和转运。我们研究了膜结构以及分子电荷和极性如何影响吸附和转运,以及抗菌化合物如何改变细菌的膜通透性。最后,我们讨论了SHS作为一种成像方式的适应性,以量化单个活细胞亚细胞区域中的分子吸附和转运。
更新日期:2019-12-11
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