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Podoviridae bacteriophage for the biocontrol of Pseudomonas aeruginosa in rainwater
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2019-11-13 , DOI: 10.1039/c9ew00896a Brandon Reyneke 1, 2, 3, 4, 5 , Sehaam Khan 5, 6, 7, 8 , Pilar Fernández-Ibáñez 9, 10, 11, 12, 13 , Wesaal Khan 1, 2, 3, 4, 5
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2019-11-13 , DOI: 10.1039/c9ew00896a Brandon Reyneke 1, 2, 3, 4, 5 , Sehaam Khan 5, 6, 7, 8 , Pilar Fernández-Ibáñez 9, 10, 11, 12, 13 , Wesaal Khan 1, 2, 3, 4, 5
Affiliation
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Bacteriophages targeting Pseudomonas spp. were isolated and characterised for the biocontrol pre-treatment of rainwater. Bacteriophages PAW33 and PFW25 were characterised as members of the Podoviridae and Myoviridae families, respectively. Bacteriophage PAW33 displayed specific activity against Pseudomonas aeruginosa (P. aeruginosa) strains and was applied in small-scale bacteriophage pre-treatment trials (8 h and 24 h) to restrict the proliferation of an environmental P. aeruginosa S1 68 strain. Hereafter, the respective samples (bacteriophage pre-treated and non-pre-treated samples) were subjected to treatment in small-scale SODIS compound parabolic collector (SODIS-CPC) systems for 4 h under natural sunlight. For the 8 h pre-treatment and SODIS-CPC trials, similar total log reductions in colony forming units (CFU) per mL and gene copies (GC) per mL were obtained for the bacteriophage pre-treated [3.68 log (CFU) and 2.34 log (GC)] and non-pre-treated [3.74 log (CFU) and 2.33 log (GC)] samples. In contrast, for the 24 h trial (followed by SODIS) a higher CFU per mL log reduction was recorded for the pre-treated sample (4.61 log) in comparison to the non-pre-treated sample (3.91 log), with comparable results obtained using EMA-qPCR. Gene expression analysis indicated that PAW33 pre-treatment for 24 h influenced the ability of P. aeruginosa S1 68 to initiate stress response mechanisms (decreased expression of the recA and lexA genes) during the SODIS-CPC treatment and resulted in the decreased expression of the phzM gene (virulence factor responsible for pyocyanin production). Bacteriophage PAW33 thus displays promise as a pre-treatment strategy of rainwater as it restricts the proliferation of P. aeruginosa and may increase the efficiency of primary disinfection methods.
中文翻译：
猪痘病毒噬菌体对雨水中的铜绿假单胞菌的生物防治
靶向噬菌假单胞菌的噬菌体。分离并表征用于雨水的生物防治预处理。噬菌体PAW33和PFW25分别被表征为Podoviridae和Myoviridae家族的成员。噬菌体PAW33对铜绿假单胞菌（P. aeruginosa）菌株具有特异性活性，并已在小型噬菌体预处理试验（8小时和24小时）中应用，以限制环境铜绿假单胞菌的增殖。S1 68株。此后，将各个样品（经过噬菌体预处理和未经预处理的样品）在小型SODIS复合抛物线收集器（SODIS-CPC）系统中在自然阳光下进行4 h处理。对于8小时的预处理和SODIS-CPC试验，经过噬菌体预处理的菌落形成单位（CFU）/ mL和基因拷贝（GC）/ mL的总对数减少量相似，分别为[3.68 log（CFU）和2.34 log（GC）]和未经预处理的[3.74 log（CFU）和2.33 log（GC）]样品。相反，对于24小时试验（紧随SODIS之后），与未预处理样品（3.91 log）相比，预处理样品（4.61 log）记录的每毫升log降低的CFU更高。使用EMA-qPCR获得。铜绿假单胞菌S1 68在SODIS-CPC处理过程中启动了应激反应机制（recA和lexA基因的表达降低），并导致phzM基因（导致黄绿色素生成的毒力因子）表达降低。因此，噬菌体PAW33有望作为雨水的预处理策略，因为它限制了铜绿假单胞菌的繁殖，并可能提高主要消毒方法的效率。
更新日期：2019-11-13
中文翻译：
猪痘病毒噬菌体对雨水中的铜绿假单胞菌的生物防治
靶向噬菌假单胞菌的噬菌体。分离并表征用于雨水的生物防治预处理。噬菌体PAW33和PFW25分别被表征为Podoviridae和Myoviridae家族的成员。噬菌体PAW33对铜绿假单胞菌（P. aeruginosa）菌株具有特异性活性，并已在小型噬菌体预处理试验（8小时和24小时）中应用，以限制环境铜绿假单胞菌的增殖。S1 68株。此后，将各个样品（经过噬菌体预处理和未经预处理的样品）在小型SODIS复合抛物线收集器（SODIS-CPC）系统中在自然阳光下进行4 h处理。对于8小时的预处理和SODIS-CPC试验，经过噬菌体预处理的菌落形成单位（CFU）/ mL和基因拷贝（GC）/ mL的总对数减少量相似，分别为[3.68 log（CFU）和2.34 log（GC）]和未经预处理的[3.74 log（CFU）和2.33 log（GC）]样品。相反，对于24小时试验（紧随SODIS之后），与未预处理样品（3.91 log）相比，预处理样品（4.61 log）记录的每毫升log降低的CFU更高。使用EMA-qPCR获得。铜绿假单胞菌S1 68在SODIS-CPC处理过程中启动了应激反应机制（recA和lexA基因的表达降低），并导致phzM基因（导致黄绿色素生成的毒力因子）表达降低。因此，噬菌体PAW33有望作为雨水的预处理策略，因为它限制了铜绿假单胞菌的繁殖，并可能提高主要消毒方法的效率。
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