The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10− adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10− counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF‐κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog‐mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.

中文翻译：

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CD10 表达识别具有高增殖和钙化潜力的人类血管周围祖细胞亚群

外膜包裹着动脉和静脉，并含有参与血管重塑的假定间充质干细胞 (MSCs)。我们在这里展示了人类外膜细胞的一个子集表达 CD10/CALLA 细胞表面金属蛋白酶。在培养中扩增时，CD10+ 和 CD10- 外膜细胞均表现出 MSCs 的表型特征。然而，与 CD10- 对应物相比，CD10+ 外膜细胞表现出更高的增殖、克隆形成和成骨潜力。CD10+ 外膜细胞通过 ERK1/2 信号传导增加细胞周期蛋白 CCND2 的表达，并通过 NF-κB 信号传导增加成骨细胞基因的表达。CD10 表达在外膜细胞中通过 sonic Hedgehog 介导的 GLI1 信号传导上调。这些结果表明，CD10、它标志着其他正常和恶性细胞谱系中快速分裂的细胞，在血管周围 MSC 功能和细胞命运规范中发挥作用。这些发现还指出了 CD10+ 血管周围细胞在血管重塑和钙化中的作用。