Bile acid (BA) imbalance may be directly associated with gastric cancer and indirectly influence stomach carcinogenesis via overexpression of histidine decarboxylase (HDC), which converts histidine (His) into histamine (HIST). Moreover, the progression of gastric cancer, could change the gut microbiome, including bacteria spp. that produce secondary BAs. Gastric juice has various metabolites that could indicate gastric cancer-related stomach conditions. Therefore, profiling of HIST, His, and BAs in gastric juice is crucial for understanding the etiological mechanisms of gastric cancer. We used a profiling method to simultaneously determine targeted metabolites in gastric juice using liquid chromatography-tandem mass spectrometry (LC-MS/MS). We successfully analyzed 70 human gastric juice samples from patients with chronic superficial gastritis (CSG, n = 20), intestinal metaplasia (IM, n = 12), and gastric cancer (n = 38). Furthermore, we investigated the relevance between BA metabolism and gastric cancer. There were statistical differences in the metabolism of cholic acid (CA) into deoxycholic acid (DCA) based on the progression of CSG into IM and gastric cancer. Hence, the progression of gastric cancer might be related to the alterations in gut microbiome composition. We provide insight into the etiological mechanisms of the progression of gastric cancer and biomarkers to diagnose and treat gastric cancer.

中文翻译：

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使用LC-MS / MS通过分析胃液中的组胺，组氨酸和胆汁酸来评估胃癌的进展。

胆汁酸（BA）失衡可能与胃癌直接相关，并通过组氨酸脱羧酶（HDC）的过表达间接影响胃癌的发生，该酶将组氨酸（His）转化为组胺（HIST）。而且，胃癌的进展，可能会改变肠道微生物组，包括细菌spp。产生二级BA。胃液中含有多种代谢物，可能表明与胃癌有关的胃部疾病。因此，分析胃液中的HIST，His和BAs对了解胃癌的病因机制至关重要。我们使用了一种分析方法，同时使用液相色谱-串联质谱法（LC-MS / MS）同时测定了胃液中的目标代谢物。我们成功地分析了来自慢性浅表性胃炎（CSG，n = 20），肠化生（IM，n = 12）和胃癌（n = 38）患者的70份人胃液样品。此外，我们调查了BA代谢与胃癌之间的相关性。根据CSG向IM和胃癌的进展，胆酸（CA）向脱氧胆酸（DCA）的代谢存在统计学差异。因此，胃癌的进展可能与肠道微生物组组成的改变有关。我们提供了胃癌进展的病因机制和诊断和治疗胃癌的生物标志物的见解。根据CSG向IM和胃癌的进展，胆酸（CA）向脱氧胆酸（DCA）的代谢存在统计学差异。因此，胃癌的进展可能与肠道微生物组组成的改变有关。我们提供了胃癌进展的病因机制和诊断和治疗胃癌的生物标志物的见解。根据CSG向IM和胃癌的进展，胆酸（CA）向脱氧胆酸（DCA）的代谢存在统计学差异。因此，胃癌的进展可能与肠道微生物组组成的改变有关。我们提供了胃癌进展的病因机制和诊断和治疗胃癌的生物标志物的见解。