A bioreactor was designed to provide high gas mass transfer to reach cell and product titres in the g L-1 level from CO2 for realistic, laboratory scale, engineered autotrophic strain evaluation. The design was based on independent CO2, H2 and air inputs and the ability to operate at high pressures. The bioreactor configuration and cultivation strategy enabled growth of Cupriavidus necator strains for long periods, to reach over 3 g L-1 dry cell weight. No negative impact of the high pressure was observed on viability of the strains up to more than 4 bar overpressure. The cultivation was then carried out using an engineered isopropanol producing strain; in this case, 3.5 g L-1 isopropanol was obtained from CO2 as the sole carbon source. This is the first reported demonstration of a successful production from engineered bacteria of product in the g L-1 range on CO2, raising the prospect of future development of CO2-based bioprocesses.

中文翻译：

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在加压生物反应器中从Cupriavidus necator中的二氧化碳生产异丙醇

生物反应器旨在提供高气体质量传递，以从 CO2 达到 g L-1 水平的细胞和产品滴度，用于实际实验室规模的工程自养菌株评估。该设计基于独立的 CO2、H2 和空气输入以及在高压下运行的能力。生物反应器的配置和培养策略能够使 Cupriavidus necator 菌株长期生长，达到超过 3 g L-1 干细胞重量。没有观察到高压对高达超过 4 巴超压的菌株的生存能力产生负面影响。然后使用工程化的产生异丙醇的菌株进行培养；在这种情况下，从作为唯一碳源的 CO2 中获得了 3.5 g L-1 异丙醇。