Elucidating structure-function relationships governing the interfacial response of human mesenchymal stem cells to polydopamine coatings.,Journal of Materials Chemistry B

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Elucidating structure-function relationships governing the interfacial response of human mesenchymal stem cells to polydopamine coatings.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-11-11 , DOI: 10.1039/c9tb02188d
Alexander J Steeves ₁ , Fabio Variola ₂

Affiliation

Deposition of mussel-inspired polydopamine (PDA) has rapidly emerged as a simple yet effective strategy to functionalize the surface of biomaterials. The experimental simplicity of the deposition process, combined with native bioactivity and bioadhesive properties, make PDA an attractive solution for biomedical applications, ranging from functional biomaterials for tissue engineering to antibacterial surfaces. Unveiling the interplay among deposition parameters, physicochemical properties of the resulting structures and their functions, is a fundamental aspect to unlock a more sophisticated knowledge of PDA biofunctionalization and its role in controlling key biological events, such as stem cell response. Although the mechanism for the bioinductive capacity of PDA is not fully understood, surface topography, chemistry and adhesive properties are believed to play a critical role, either individually or in combination. This work addresses the differential roles of such surface properties on PDA bioactivity. We achieved novel insights on the physicochemical makeup of two PDA coatings obtained by varying one critical parameter (i.e., solution agitation) during the deposition. Successively, we focused on the effects on human mesenchymal stem cells (hMSCs) in both normal and serum-free culturing conditions. This study reveals both the serum-dependent and independent cueing involved in bioactive induction caused by PDA.

中文翻译：

阐明控制人间充质干细胞对聚多巴胺涂层的界面反应的结构-功能关系。

贻贝启发性聚多巴胺（PDA）的沉积已迅速出现，成为一种简单而有效的策略，可对生物材料的表面进行功能化。沉积过程的实验简便性与天然的生物活性和生物粘附特性相结合，使PDA成为生物医学应用（从用于组织工程的功能性生物材料到抗菌表面）的有吸引力的解决方案。揭示沉积参数，所得结构的理化性质及其功能之间的相互作用是解锁PDA生物功能化及其在控制关键生物事件（如干细胞反应）中的作用的更复杂知识的基本方面。尽管尚未完全了解PDA的生物感应能力的机制，但表面形貌，无论是单独还是组合使用，化学和粘合性能都起着至关重要的作用。这项工作解决了这种表面性质对PDA生物活性的不同作用。我们通过改变一个关键参数获得了两种PDA涂层的理化组成的新颖见解（即，在沉积过程中溶液中搅动）。继而，我们专注于在正常和无血清培养条件下对人间充质干细胞（hMSCs）的影响。这项研究揭示了由PDA引起的生物活性诱导中涉及的血清依赖性和独立性提示。

更新日期：2019-11-11

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