BACKGROUND AND AIM Sodium nitrite (NaNO2) is an inorganic salt with numerous applications in a variety of industries, as well as in medicine. Nevertheless, exposure to high levels of NaNO2 is toxic for animals and humans. Sodium nitrite intoxication is shown to decrease the activity of major antioxidant defence enzymes which is dependent on the maintenance of specific ion equilibrium. The aim of the present study was to investigate the effect of acute NaNO2 intoxication on the content of the essential metals iron (Fe), calcium (Ca) and zinc (Zn) in mouse spleen. METHODS Mature male ICR mice were divided into four groups and subjected to acute NaNO2 exposure by a single intraperitoneal injection of 120 mg/kg body weight. Animals in each group were sacrificed at certain time interval after treatment (1 h, 5 h, 1 day and 2 days). Spleens were excised and processed for atomic absorption spectrometry analysis of Fe, Ca and Zn content. RESULTS At the first hour after treatment, a decrease in Fe and Ca levels was observed. One day following NaNO2 administration, Zn concentration reached its lowest value and Ca levels remained lower, compared to the untreated controls. In contrast, Fe concentration increased on the first and second day after treatment. CONCLUSION The results of the present study demonstrate that acute NaNO2 intoxication provokes changes in the endogenous levels of Fe, Ca and Zn in mouse spleen. These findings suggest disruption of the ionic balance and impact on the activity of antioxidant defence enzymes.

中文翻译：

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急性亚硝酸钠中毒对小鼠脾脏中某些必需生物金属的影响。

背景与目的亚硝酸钠（NaNO2）是一种无机盐，在许多行业以及医学中都有广泛的应用。但是，暴露于高水平的NaNO2对动物和人类都是有毒的。亚硝酸钠中毒显示会降低主要抗氧化剂防御酶的活性，这取决于维持特定离子平衡。本研究的目的是研究急性NaNO2中毒对小鼠脾脏中必需金属铁（Fe），钙（Ca）和锌（Zn）含量的影响。方法将成年雄性ICR小鼠分为四组，并通过单次腹膜内注射120 mg / kg体重进行急性NaNO2暴露。在治疗后的一定时间间隔（1小时，5小时，1天和2天）处死每组的动物。切除脾脏并进行处理，以进行铁，钙和锌含量的原子吸收光谱分析。结果在治疗后的第一小时，观察到Fe和Ca水平下降。与未处理的对照组相比，施用NaNO2一天后，Zn的浓度达到最低值，而Ca的含量仍然较低。相反，在治疗后的第一天和第二天，铁浓度增加。结论本研究的结果表明，急性NaNO2中毒可引起小鼠脾脏内源性Fe，Ca和Zn含量的变化。这些发现表明离子平衡的破坏以及对抗氧化防御酶活性的影响。观察到Fe和Ca水平降低。与未处理的对照组相比，施用NaNO2一天后，Zn的浓度达到最低值，而Ca的含量仍然较低。相反，在治疗后的第一天和第二天，铁浓度增加。结论本研究的结果表明，急性NaNO2中毒可引起小鼠脾脏内源性Fe，Ca和Zn含量的变化。这些发现表明离子平衡的破坏以及对抗氧化防御酶活性的影响。观察到Fe和Ca水平降低。与未处理的对照组相比，施用NaNO2一天后，Zn的浓度达到最低值，而Ca的含量仍然较低。相反，在治疗后的第一天和第二天，铁浓度增加。结论本研究的结果表明，急性NaNO2中毒可引起小鼠脾脏内源性Fe，Ca和Zn含量的变化。这些发现表明离子平衡的破坏以及对抗氧化防御酶活性的影响。结论本研究的结果表明，急性NaNO2中毒可引起小鼠脾脏内源性Fe，Ca和Zn含量的变化。这些发现表明离子平衡的破坏以及对抗氧化防御酶活性的影响。结论本研究的结果表明，急性NaNO2中毒可引起小鼠脾脏内源性Fe，Ca和Zn含量的变化。这些发现表明离子平衡的破坏以及对抗氧化防御酶活性的影响。