Gastric cancer is a malignancy of very poor prognosis and survival rates. Macrocalyxin C is a Chinese herb-derived diterpenoid compound that has been postulated to possess anti-cancer characteristics. Gastic cell viability and stage of cell cycle were assessed using CCK8 assay and flow cytometry, respectively. Cell migration and invation were assessed using the wound healing and Transwell assays. Rate of apoptosis was determined via AV/PI-staining. Athymic nude mice xenograft models were used to evaluate the in vivo efficacy of macrocalyxin C. Western blot, luciferase experiments, cell transfection and real-time PCR allowed further study into the activation of the miR-212-3p/Sox6 pathway during macrocalyxin C treatment. We conclude that macrocalyxin C may halt the proliferation of gastric malignancies through alteration of cell invasion, apoptosis, progression through the cell cycle and cell growth. The macrocalyxin C→miR-212-3p┤Sox6 signal pathway was identified to be involved in Sox6 attenuation through augmentation of miR-212-3p values.

中文翻译：

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大萼蛋白 C 通过激活 miR-212-3p/Sox6 通路来减少胃癌的增殖和侵袭。


胃癌是一种预后和生存率极差的恶性肿瘤。 Macrocalyxin C 是一种源自中草药的二萜化合物，被认为具有抗癌特性。分别使用 CCK8 测定和流式细胞术评估胃细胞活力和细胞周期阶段。使用伤口愈合和Transwell检测评估细胞迁移和侵袭。通过AV/PI染色测定细胞凋亡率。使用无胸腺裸鼠异种移植模型评估大萼蛋白 C 的体内功效。蛋白质印迹、荧光素酶实验、细胞转染和实时 PCR 可以进一步研究大萼蛋白 C 治疗期间 miR-212-3p/Sox6 通路的激活。我们得出的结论是，大萼蛋白 C 可能通过改变细胞侵袭、细胞凋亡、细胞周期进展和细胞生长来阻止胃恶性肿瘤的增殖。大萼蛋白 C→miR-212-3p┤Sox6 信号通路被确定通过增强 miR-212-3p 值参与 Sox6 衰减。