A polyhydroxyalkanoate-based encapsulating strategy for 'bioplasticizing' microorganisms.,Microbial Biotechnology

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A polyhydroxyalkanoate-based encapsulating strategy for 'bioplasticizing' microorganisms.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2019-11-12 , DOI: 10.1111/1751-7915.13492
Erika González _{1,

2} , Cristina Herencias _{1,

2} , M Auxiliadora Prieto _{1,

2}

Affiliation

Over the past few decades, considerable interest has been shown in developing nano‐ and microcarriers with biocompatible and biodegradable materials for medical and biotechnological applications. Microencapsulation is a technology capable of enhancing the survival rate of bacteria, providing stability in harsh environments. In the present paper, we developed a technology to encapsulate microorganisms within polyhydroxyalkanoate (PHA)‐based microcapsules (MPs), employing a modified double emulsion solvent evaporation technique, with Pseudomonas putida KT2440 as a biotechnological model strain. The resulting MPs display a spherical morphology and an average particle size of 10 μm. The stability of the MPs was monitored under different conditions of storage and stress. The MPs remained stable for at least 24 days stored at 4°C in a water suspension. They exhibited greater tolerance to stress conditions; encapsulated cells remained viable for 2 h in alkaline solution and after 24 h of H₂O₂ exposure at 10 and 20 mM. Results suggested the potential of MPs as a microcontainer of bacterial cells, even for biotechnological applications requiring high alkaline conditions and oxidative stress. We validated the potential applicability of the PHA‐based microencapsulation method in other microorganisms by encapsulating the predatory bacterium Bdellovibrio bacteriovorus.

中文翻译：

一种用于“生物增塑”微生物的基于聚羟基烷酸酯的封装策略。

在过去的几十年里，人们对开发用于医疗和生物技术应用的具有生物相容性和可生物降解材料的纳米和微载体表现出了极大的兴趣。微胶囊化是一种能够提高细菌存活率，在恶劣环境中提供稳定性的技术。在本文中，我们开发了一种将微生物封装在基于聚羟基链烷酸酯 (PHA) 的微胶囊 (MPs) 中的技术，采用改进的双乳液溶剂蒸发技术，与恶臭假单胞菌KT2440 作为生物技术模型菌株。所得 MP 呈现球形形态，平均粒径为 10 μm。在不同的储存和压力条件下监测 MPs 的稳定性。MPs 在 4°C 的水悬浮液中储存至少 24 天保持稳定。他们对压力条件表现出更大的耐受性；封装的细胞在碱性溶液中保持存活 2 小时，在 10 和 20 mM的 H ₂ O _{2暴露 24 小时后保持存活。}结果表明 MPs 具有作为细菌细胞微容器的潜力，即使对于需要高碱性条件和氧化应激的生物技术应用也是如此。我们通过包裹捕食性细菌验证了基于 PHA 的微囊化方法在其他微生物中的潜在适用性Bdellovibrio bacteriovorus。

更新日期：2019-11-12

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