An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids.,Nature Nanotechnology

当前位置： X-MOL 学术 › Nat. Nanotechnol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids.
Nature Nanotechnology ( IF 38.1 ) Pub Date : 2019-11-11 , DOI: 10.1038/s41565-019-0566-z
Jonathan Sabaté Del Río _{1,

2} , Olivier Y F Henry _{1,

3} , Pawan Jolly ₁ , Donald E Ingber _{1,

4,

5}

Affiliation

Affinity-based electrochemical detection in complex biological fluids could enable multiplexed point-of-care diagnostics for home healthcare; however, commercialization of point-of-care devices has been limited by the rapid loss of sensitivity caused by electrode surface inactivation and biofouling. Here, we describe a simple and robust antifouling coating for electrodes consisting of a three-dimensional porous matrix of cross-linked bovine serum albumin supported by a network of conductive nanomaterials composed of either gold nanowires, gold nanoparticles or carbon nanotubes. These nanocomposites prevent non-specific interactions while enhancing electron transfer to the electrode surface, preserving 88% of the original signal after 1 month of exposure to unprocessed human plasma, and functionalization with specific antibodies enables quantification of anti-interleukin 6 in plasma with high sensitivity. The easy preparation, stability and simplicity of this nanocomposite allow the generation of electrochemical biosensors that can operate in complex biological fluids such as blood plasma or serum.

中文翻译：

一种防污涂层，可在复杂的生物流体中实现基于亲和力的电化学生物传感。

在复杂的生物流体中基于亲和力的电化学检测可以实现家庭医疗保健的多重即时诊断。然而，由于电极表面失活和生物污染而导致灵敏度的快速下降，限制了即时医疗器械的商业化。在这里，我们描述了一种用于电极的简单而坚固的防污涂料，该涂料由交联的牛血清白蛋白的三维多孔基质组成，并由由金纳米线，金纳米颗粒或碳纳米管组成的导电纳米材料网络支撑。这些纳米复合材料可防止非特异性相互作用，同时增强电子向电极表面的转移，在暴露于未经处理的人体血浆中1个月后，可保留原始信号的88％，通过特异性抗体的功能化，可以高灵敏度地定量血浆中的抗白介素6。这种纳米复合材料的易于制备，稳定性和简单性允许生成可在复杂生物流体（例如血浆或血清）中运行的电化学生物传感器。

更新日期：2019-11-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11