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Arabidopsis PIC30 encodes a major facilitator superfamily transporter responsible for the uptake of picolinate herbicides.
The Plant Journal ( IF 6.2 ) Pub Date : 2020-02-11 , DOI: 10.1111/tpj.14608
Praveen K Kathare 1, 2 , Sunethra Dharmasiri 1 , Eric D Vincill 3 , Pratyush Routray 3 , Idrees Ahmad 1 , Daniel M Roberts 3 , Nihal Dharmasiri 1
Affiliation  

Picloram is an auxinic herbicide that is widely used for controlling broad leaf weeds. However, its mechanism of transport into plants is poorly understood. In a genetic screen for picloram resistance, we identified three Arabidopsis mutant alleles of PIC30 (PICLORAM RESISTANT30) that are specifically resistant to picolinates, but not to other auxins. PIC30 is a previously uncharacterized gene that encodes a major facilitator superfamily (MFS) transporter. Similar to most members of MFS, PIC30 contains 12 putative transmembrane domains, and PIC30-GFP fusion protein selectively localizes to the plasma membrane. In planta transport assays demonstrate that PIC30 specifically transports picloram, but not indole-3-acetic acid (IAA). Functional analysis of Xenopus laevis oocytes injected with PIC30 cRNA demonstrated PIC30 mediated transport of picloram and several anions, including nitrate and chloride. Consistent with these roles of PIC30, three allelic pic30 mutants are selectively insensitive to picolinate herbicides, while pic30-3 is also defective in chlorate (analogue of nitrate) transport and also shows reduced uptake of 15 N O 3 - . Overexpression of PIC30 fully complements both picloram and chlorate insensitive phenotypes of pic30-3. Despite the continued use of picloram as an herbicide, a transporter for picloram was not known until now. This work provides insight into the mechanisms of plant resistance to picolinate herbicides and also shed light on the possible endogenous function of PIC30 protein.

中文翻译:

拟南芥PIC30编码负责摄取吡啶甲酸类除草剂的主要促进子超家族转运蛋白。

Picloram是一种生长素除草剂,广泛用于防治阔叶杂草。但是,对其转运到植物中的机制了解甚少。在对吡喃类抗生素抗性的遗传筛选中,我们鉴定出了PIC30的三个拟南芥突变体等位基因(PICLORAM RESISTANT30),它们对吡啶甲酸酯类(而非其他植物生长素)具有特异性抗性。PIC30是一个以前未鉴定的基因,编码主要的促进子超家族(MFS)转运蛋白。与MFS的大多数成员相似,PIC30包含12个推定的跨膜结构域,而PIC30-GFP融合蛋白选择性定位在质膜上。在植物中的转运分析表明,PIC30专门转运了吡咯仑,但没有转运吲哚-3-乙酸(IAA)。注射PIC30 cRNA的非洲爪蟾卵母细胞的功能分析表明,PIC30介导了吡咯烷和几种阴离子(包括硝酸根和氯离子)的转运。与PIC30的这些作用一致,三个等位基因pic30突变体对吡啶甲酸除草剂选择性不敏感,而pic30-3在氯酸盐(硝酸盐的类似物)运输中也有缺陷,并且显示出对15 NO 3-的吸收减少。PIC30的过表达完全补充了pic30-3的吡咯仑和氯酸盐不敏感表型。尽管继续使用吡咯兰姆作为除草剂,但到目前为止还不知道吡咯兰的转运蛋白。这项工作提供了对植物抗吡啶甲酸除草剂的机制的见解,也阐明了PIC30蛋白可能的内源功能。与PIC30的这些作用一致,三个等位基因pic30突变体对吡啶甲酸除草剂选择性不敏感,而pic30-3在氯酸盐(硝酸盐的类似物)运输中也有缺陷,并且显示出对15 NO 3-的吸收减少。PIC30的过表达完全补充了pic30-3的吡咯仑和氯酸盐不敏感表型。尽管继续使用吡咯兰姆作为除草剂,但到目前为止还不知道吡咯兰的转运蛋白。这项工作提供了对植物抗吡啶甲酸除草剂的机制的见解,也阐明了PIC30蛋白可能的内源功能。与PIC30的这些作用一致,三个等位基因pic30突变体对吡啶甲酸除草剂选择性不敏感,而pic30-3在氯酸盐(硝酸盐的类似物)运输中也有缺陷,并且显示出对15 NO 3-的吸收减少。PIC30的过表达完全补充了pic30-3的吡咯仑和氯酸盐不敏感表型。尽管继续使用吡咯兰姆作为除草剂,但到目前为止还不知道吡咯兰的转运蛋白。这项工作提供了对植物抗吡啶甲酸除草剂的机制的见解,也阐明了PIC30蛋白可能的内源功能。PIC30的过表达完全补充了pic30-3的吡咯仑和氯酸盐不敏感表型。尽管继续使用吡咯兰姆作为除草剂,但到目前为止还不知道吡咯兰的转运蛋白。这项工作提供了对植物抗吡啶甲酸除草剂的机制的见解,也阐明了PIC30蛋白可能的内源功能。PIC30的过表达完全补充了pic30-3的吡咯仑和氯酸盐不敏感表型。尽管继续使用吡咯兰姆作为除草剂,但到目前为止还不知道吡咯兰的转运蛋白。这项工作提供了对植物抗吡啶甲酸除草剂的机制的见解,也阐明了PIC30蛋白可能的内源功能。
更新日期:2020-02-11
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