Force-clamp spectroscopy can mimic the physiological conditions for the proteins under investigation. In addition, it is a direct way of observing the relationship between bond lifetime and molecular forces. However, traditional force-clamp methods rely on active feedback controllers that can introduce artefacts. In this work, we introduce a new method to enable force-clamp spectroscopy without a need for an active feedback. The method is based on miniaturized magnetic beads offering improved stability. As a case study, we performed force-clamp experiments using biotin-streptavidin molecule pairs with and without active feedback. Our results demonstrate the feasibility of force-clamp experiments without feedback and illustrate the advantages of our method.

中文翻译：

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用于基于力钳光谱的单分子测量的微型磁珠致动器

力钳光谱可以模拟所研究蛋白质的生理条件。此外，它是观察键寿命和分子力之间关系的直接方法。然而，传统的力钳方法依赖于可能引入伪影的主动反馈控制器。在这项工作中，我们引入了一种无需主动反馈即可启用力钳光谱的新方法。该方法基于提供更高稳定性的微型磁珠。作为案例研究，我们使用有和没有主动反馈的生物素-链霉亲和素分子对进行了力钳实验。我们的结果证明了没有反馈的力钳实验的可行性，并说明了我们方法的优点。