Liposoluble molecules are a group of compounds that display potent biological and therapeutic properties. The present study aimed to identify liposoluble molecules produced by Ganoderma lipsiense grown in red rice medium using solid‐state fermentation (SSF) techniques, and to investigate the antigiardial and antibacterial activities potential of extracts in vitro . Eighteen fatty acids and derivatives were identified by gas chromatograph‐mass spectrometry (GC‐MS) analysis in G. lipsense extract. Qualitative (Fourier transform infrared spectroscopy and nuclear magnetic resonance) characterizations identified the steroid ergosta‐6,22‐diene‐3β,5α,8α‐triol in purified hexane subfraction (HEXsf ) F19 isolated from hexane fraction (HEXf ) of crude extract (CE ). Ergosta‐6,22‐diene‐3β,5α,8α‐triol exhibited significant inhibitory activity against Giardia duodenalis throphozoites (93.6%) in in vitro assays. CE and HEXf inhibited 95.38% and 92.74% of the G. duodenalis throphozoites in 100 µg mL⁻¹, whereas CE and their fractions dichloromethane (DCMf ) and ethyl acetate (EAf ) showed antibacterial activities against Pseudomonas aeruginosa and Staphylococcus aureus at 500 µg mL⁻¹. Importantly, some liposoluble compounds produced and identified in G. lipsiense are unpublished for this species. This is first report for the production of ergosta‐6,22‐diene‐3β,5α,8α‐triol by G. lipsiense and its antiparasitic activity.

中文翻译：

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脂灵芝的脂溶性化合物在具有抗寄生虫和抗菌特性的固体红米培养基上生长。

脂溶性分子是一组显示出强大的生物学和治疗特性的化合物。本研究旨在鉴定使用固态发酵（SSF）技术在红米培养基中生长的脂灵芝产生的脂溶性分子，并研究提取物的体外抗细菌和抗菌活性。气相色谱-质谱法（GC-MS）分析鉴定了唇香提取物中的十八种脂肪酸及其衍生物。定性（傅立叶变换红外光谱和核磁共振）表征确定了纯正己烷亚组分（HEXsf）F19中的类固醇ergosta-6,22-diene-3β，5α，8α-三醇从粗提取物（CE）的己烷馏分（HEXf）中分离得到。在体外试验中， Ergosta-6,22-diene-3β，5α，8α-三醇显示出对十二指肠贾第鞭毛虫的滋养体的显着抑制活性（93.6％）。CE和HEXf在100 µg mL ^-1中抑制了十二指肠亚种的滋养体的95.38％和92.74％，而CE及其组分二氯甲烷（DCMf）和乙酸乙酯（EAf）在500 µg mL时显示出对铜绿假单胞菌和金黄色葡萄球菌的抗菌活性。^-1。重要的是，尚没有公开在唇齿肠球菌中产生和鉴定的一些脂溶性化合物。这是通过产生麦角甾6,22二烯3β，5α，8α三醇的第一报告G. lipsiense及其抗寄生虫活性。