Abstract Tuberculosis (TB) is a chronic infectious disease that usually affects the pulmonary region of human beings. It remains critically important to develop cost effective methods for TB detection, especially since impoverished communities are often the target population. Immunological assays are commonly used for the detection of TB and are typically based on selected antibody (Ab) antigen (Ag) interactions as measured by the enzyme-linked immunosorbent assay (ELISA). It is also common to have tagged antibody/antigen binding pairs where the signaling is produced by the tag as is the case with the indirect tuberculosis IgG ELISA Kit, where a horseradish peroxidase (HRP) tag on the antibody is responsible for the analytical signal. Mycobacterium tuberculosis (MTB), recombinant protein antigen 85B (Ag85B), is the most abundant protein exposed by Mycobacterium tuberculosis acting as a potent immuno-protective antigen. In this work, both Ag85B and antibody-HRP (Ab-HRP) were immobilized on a semi-conductive polyamic acid (PAA) electrochemical interface towards the design of new electrochemical sensors. In a first approach, the antigen, Ag85B, was employed as the bioreceptor to afford label-free TB signaling through antibody detection and the obtained limit of detection was 8 μg/mL of antibody. The response was evaluated by voltammetric methods and electron impedance spectroscopy (EIS). Cyclic voltammetry (CV) and EIS were used to characterize the charge transfer and capacitive behavior of the label-free sensor. In a second approach, the HRP tagged antibody was used for the design of a stable hydrogen peroxide (H2O2) sensor with very good analytical performance with a limit of detection equal to 1.3 μM. Even though we developed an ultrasensitive H2O2 sensor, no further development of TB antigen detection was possible in this work.

中文翻译：

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固定在玻璃碳电极 (GCE) 上的聚酰胺酸 (PAA) 作为用于感测结核病 (TB) 抗体和测定过氧化氢的电化学平台

摘要 结核病（TB）是一种慢性传染病，通常影响人类的肺部区域。开发具有成本效益的结核病检测方法仍然至关重要，特别是因为贫困社区通常是目标人群。免疫学检测通常用于检测 TB，通常基于选择的抗体 (Ab) 抗原 (Ag) 相互作用，如酶联免疫吸附检测 (ELISA) 所测量。带有标签的抗体/抗原结合对也很常见，其中信号由标签产生，就像间接结核病 IgG ELISA 试剂盒的情况一样，其中抗体上的辣根过氧化物酶 (HRP) 标签负责分析信号。结核分枝杆菌 (MTB)、重组蛋白抗原 85B (Ag85B)、是结核分枝杆菌暴露的最丰富的蛋白质，作为一种有效的免疫保护抗原。在这项工作中，Ag85B 和抗体-HRP (Ab-HRP) 都固定在半导体聚酰胺酸 (PAA) 电化学界面上，以设计新的电化学传感器。在第一种方法中，抗原 Ag85B 用作生物受体，通过抗体检测提供无标记的 TB 信号，获得的检测限为 8 μg/mL 抗体。通过伏安法和电子阻抗谱 (EIS) 评估响应。循环伏安法 (CV) 和 EIS 用于表征无标记传感器的电荷转移和电容行为。在第二种方法中，HRP 标记抗体用于设计稳定的过氧化氢 (H2O2) 传感器，该传感器具有非常好的分析性能，检测限等于 1.3 μM。尽管我们开发了一种超灵敏的 H2O2 传感器，但在这项工作中不可能进一步开发 TB 抗原检测。