Abstract A two-photon fluorescent probe has been adopted for bisulfite based on an intramolecular charge transfer (ICT) mechanism. The fluorescence intensity of the probe decreased by 837 fold following treatment with HSO3− and the detection limit was 42 nM. This probe showed excellent properties such as high photostability, good two-photon properties, and large Stokes shifts. More importantly, this approach offers a rapid, highly selective, and sensitive method to determine HSO3− in buffer solutions and real samples. Furthermore, the probe was successfully used for two-photon fluorescence visualization of trace SO2 derivatives in biological systems.

中文翻译：

---

基于分子内电荷转移 (ICT) 的双光子荧光探针，用于具有生物成像应用的亚硫酸氢盐

摘要 基于分子内电荷转移（ICT）机制，已采用双光子荧光探针检测亚硫酸氢盐。用 HSO3- 处理后，探针的荧光强度降低了 837 倍，检测限为 42 nM。该探针显示出优异的特性，例如高光稳定性、良好的双光子特性和大斯托克斯位移。更重要的是，这种方法提供了一种快速、高选择性和灵敏的方法来测定缓冲溶液和实际样品中的 HSO3−。此外，该探针已成功用于生物系统中痕量 SO2 衍生物的双光子荧光可视化。