MeJA triggers a time-dependent behavior of the phenylpropanoid compounds. Plant cells produce a large number of metabolites in response to environmental factors. The cellular responses to environmental changes are orchestrated by signaling molecules, such as methyl jasmonate (MeJA). To understand how the MeJA changes the behavior of amino acids, carbohydrates, and phenylpropanoid compounds such as phenolic acids, phenylethanoid-glycosides, and flavonoids in Scrophularia striata cells; we monitored the metabolic responses for different times of exposure. In this study, we performed a time course analysis of metabolites and enzymes in S. striata cells exposed to MeJA (100 µM) and evaluated the metabolic flux towards carbon-rich secondary metabolites production. Moreover, we calculated the biosynthetic energy cost for free amino acids. Our results indicated that MeJA accelerates the sucrose degradation and directs the metabolic fluxes towards a pool of flavonoids and phenylethanoid glycosides through a change in enzyme behavior in the entry point and center of the phenylpropanoid pathway. MeJA also decreased and then raised the amino acid biosynthesis cost in S. striata cells in a time-dependent manner, indicating the cells evolve to utilize amino acids more economically by reducing cell growth. Finally, we classified the marked changes in the metabolites level and enzyme activities into three groups including early-, late-, and oscillatory-response groups to MeJA and summarized our findings as a model depicting pathway interactions during MeJA elicitation. Determination of metabolic levels in response to MeJA suggests that the changes in metabolic responses are time-dependent.

中文翻译：

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在玄参细胞培养物中苯丙烷途径响应茉莉酸甲酯的时间依赖性行为。

MeJA 触发苯丙烷化合物的时间依赖性行为。植物细胞响应环境因素产生大量代谢物。细胞对环境变化的反应是由信号分子协调的，例如茉莉酸甲酯 (MeJA)。了解 MeJA 如何改变玄参细胞中氨基酸、碳水化合物和苯丙烷化合物（如酚酸、苯乙醇苷和类黄酮）的行为；我们监测了不同暴露时间的代谢反应。在这项研究中，我们对暴露于 MeJA (100 µM) 的 S. striata 细胞中的代谢物和酶进行了时间进程分析，并评估了向富含碳的次级代谢物产生的代谢通量。此外，我们计算了游离氨基酸的生物合成能量成本。我们的研究结果表明，MeJA 通过改变苯丙烷途径入口点和中心的酶行为，加速蔗糖降解，并将代谢通量引导至黄酮类和苯乙醇苷池。MeJA 还以时间依赖性方式降低然后提高了 S. striata 细胞中的氨基酸生物合成成本，表明细胞通过减少细胞生长而进化为更经济地利用氨基酸。最后，我们将代谢物水平和酶活性的显着变化分为三组，包括对 MeJA 的早期、晚期和振荡反应组，并将我们的发现总结为描述 MeJA 诱导过程中通路相互作用的模型。测定响应 MeJA 的代谢水平表明代谢反应的变化是时间依赖性的。