The Hippo pathway is a key signaling pathway in the control of organ size and development. The most distal elements of this pathway, the TEAD transcription factors, are regulated by several proteins, such as YAP (Yes-associated protein), TAZ (transcriptional co-activator with PDZ-binding motif) and VGLL1-4 (Vestigial-like members 1-4). In this article, combining structural data and motif searches in protein databases, we identify two new TEAD interactors: FAM181A and FAM181B. Our structural data show that they bind to TEAD via an Ω-loop as YAP/TAZ do, but only FAM181B possesses the LxxLF motif (x any amino acid) found in YAP/TAZ. The affinity of different FAM181A/B fragments for TEAD is in the low micromolar range and full-length FAM181A/B proteins interact with TEAD in cells. These findings, together with a recent report showing that FAM181A/B proteins have a role in nervous system development, suggest a potential new involvement of the TEAD transcription factors in the development of this tissue.

中文翻译：

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将FAM181A和FAM181B鉴定为与TEAD转录因子的新相互作用物。

河马途径是控制器官大小和发育的关键信号传导途径。TEAD转录因子是该途径的最远端元件，受几种蛋白质调节，例如YAP（是，是相关蛋白质），TAZ（具有PDZ结合基序的转录共激活因子）和VGLL1-4（前庭样成员） 1-4）。在本文中，结合蛋白质数据库中的结构数据和基序搜索，我们确定了两个新的TEAD相互作用子：FAM181A和FAM181B。我们的结构数据表明，它们像YAP / TAZ一样通过Ω环与TEAD结合，但是只有FAM181B拥有LAPLF基序（x任意氨基酸）。不同FAM181A / B片段对TEAD的亲和力处于低微摩尔范围内，全长FAM181A / B蛋白与TEAD在细胞中相互作用。这些发现，