The purpose of this work was to contribute to the understanding of the mechanism of action of two new ixodicides. The histological and ultrastructural alterations of Rhipicephalus microplus oocytes (San Alfonso strain) treated with two new ethyl-carbamates (ethyl-4-bromophenyl carbamate and ethyl-4-chlorophenyl carbamate) by the adult immersion test were evaluated by light microscopy and transmission electron microscopy. The effects of the carbamates on embryogenesis in eggs were evaluated by fluorescence microscopy using DAPI staining. Both ethyl-carbamates inhibited the maturation of most oocytes and induced a concentration-dependent decrease (r² = 0.5, p < 0.05) in the embryonation percentage in the small number of eggs oviposited by treated ticks. Evident ultrastructural alterations were observed in the oocytes from ticks exposed to the ethyl-carbamates, including modification of the chorion structure, myelinic bodies and autophagic vacuoles that were associated with degenerated organelles (mitochondria, endoplasmic reticulum and yolk granules), nucleolus fragmentation and chromatin clumping in germinal vesicles. In conclusion, these ethyl-carbamates affect the reproductive potential of R. microplus due to their negative effects on oogenesis and their repercussions for embryonic development.

这项工作的目的是促进对两种新杀螨剂作用机理的理解。通过光学显微镜和透射电子显微镜评估了成人浸没试验中，用两种新的氨基甲酸乙酯（氨基甲酸4-溴苯基酯和氨基甲酸4-氯苯基乙酯）处理的小头蛇小卵母细胞（San Alfonso菌株）的组织学和超微结构改变。。氨基甲酸酯对鸡蛋中胚胎发生的影响通过使用DAPI染色的荧光显微镜进行了评估。两种氨基甲酸乙酯均抑制大多数卵母细胞的成熟并引起浓度依赖性的降低（r ²= 0.5，p <0.05）在经过处理的tick虫产卵的少量卵中的胚胎百分比。暴露于氨基甲酸乙酯的tick的卵母细胞中观察到明显的超微结构改变，包括与变性细胞器（线粒体，内质网和卵黄颗粒），绒毛膜碎裂和染色质结块有关的绒毛膜结构，髓鞘小体和自噬泡的改变。在生小泡中。总之，这些乙基氨基甲酸酯由于它们对卵子发生的不利影响及其对胚胎发育的影响，因此会影响微小红杆菌的繁殖潜能。