In the paper, a DNA assisted magnetic reduced graphene oxide-copper nanocomposite (mrGO-CuNCs) is proposed. Copper ions are able to accelerate the oxidation of o-phenylenediamine. The electrochemical signal of the oxidized product, 2,3-diaminobenazine, are employed to characterize the HCV DNAs. The strategy is able to sensitively detect HCV DNA over a wide linear range within the range of 0.5-10 nM, and has a detection limit of 405.0 pM. Critically, it could effectively discriminate the 1b and 6k subtypes of HCV. This assay strategy has promising applications in the early diagnosis and blood screening of HCV.

中文翻译：

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基于核苷酸辅助磁性还原氧化石墨烯-铜纳米复合材料的丙型肝炎病毒亚型的灵敏电化学检测

本文提出了一种DNA辅助磁性还原氧化石墨烯-铜纳米复合材料（mrGO-CuNCs）。铜离子能够加速邻苯二胺的氧化。氧化产物2，3-二氨基联苯胺的电化学信号用于表征HCV DNA。该策略能够在0.5-10 nM的宽线性范围内灵敏地检测HCV DNA，并且检测限为405.0 pM。至关重要的是，它可以有效地区分HCV的1b和6k亚型。这种测定策略在HCV的早期诊断和血液筛查中具有广阔的应用前景。