HIV-1 genetic diversity evolution was deeply characterized during the first year of infection among recently-infected patients using deep sequencing technology and correlated with disease progression surrogate markers. RNA and DNA samples from twenty-five individuals (13 female) encoding the protease and reverse transcriptase regions of the pol gene, and the V3 region of the env gene were evaluated at recent infection and during established infection. Infection by a unique HIV-1 strain was inferred in 70.1% of the individuals, with no differences between genders. Infections by multiple strains were associated with higher viral loads and faster CD4+ T cell declines. Either low or high levels of viral loads accompanied low levels of genetic diversity and lower selective pressure. With massive sequence data from 3 distinct genomic HIV-1 regions from plasma and PBMCs over time, we propose a model for HIV-1 genetic diversity, which correlates to basal viral loads of patients.

中文翻译：

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HIV-1遗传多样性和分歧及其与抗逆转录病毒新近感染个体之间疾病进展的相关性。

使用深度测序技术，在感染第一年的艾滋病毒感染者的头一年中，使用深度测序技术对HIV-1遗传多样性的演变进行了深层表征，并与疾病进展的替代指标相关。在最近的感染和确定的感染期间，评估了来自25个个体（13个女性）的RNA和DNA样本，这些个体编码pol基因的蛋白酶和逆转录酶区域以及env基因的V3区域。推断70.1％的个体感染了独特的HIV-1毒株，性别无差异。多种病毒株的感染与较高的病毒载量和较快的CD4 + T细胞下降有关。低或高水平的病毒载量伴随着低水平的遗传多样性和较低的选择压力。