Combining releasable chemical crosslinkers with photoaffinity probes represents a valuable tool for identifying protein-protein interactions (PPIs). The biomacromolecule photoaffinity probe prepared by using releasable photoaffinity linkers can be used to exploring PPIs by triggering release of the releasable group. More importantly, it can overcome the shortcomings of macromolecular photoaffinity probes without label transfer functionality to accurately confirm defects in specific structural sites. It shows particular promise for research exploring the interaction of unknown proteins and transient-weak PPIs in living organisms to discover new drug targets. In this review, we highlight recent progress in the development and application of chemical releasable linkers in photoaffinity probes. Several comparative studies are described in which the efficiency of various photoaffinity probes are compared.

中文翻译：

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基于活动的蛋白质组分析：可释放接头在光亲和探针中的应用。

将可释放的化学交联剂与光亲和探针结合在一起，可作为鉴定蛋白质-蛋白质相互作用（PPI）的有价值的工具。通过使用可释放的光亲和性接头制备的生物大分子光亲和性探针可用于通过触发可释放基团的释放来探索PPI。更重要的是，它可以克服没有标记转移功能的大分子光亲和探针的缺点，从而无法准确确认特定结构位点的缺陷。它为研究未知蛋白质与短暂的弱PPI在活生物体中的相互作用以发现新的药物靶标的研究提供了特别的希望。在这篇综述中，我们重点介绍了在光亲和探针中化学可释放接头的开发和应用的最新进展。