This study aimed to investigate the effects of teaghrelin, an active ingredient of Chin-shin oolong tea, on murine C2C12 myoblast cells. Under high serum conditions, teaghrelin inhibited C2C12 cell proliferation, indicating a cell cycle arrest and cessation of proliferative progression. Teaghrelin promoted pro-differentiation of C2C12 cells as evidenced by a progressively elongated morphology, as well as the induction of muscle specific myogenin, myosin heavy chain (MHC), and MyoD. The formation of multinucleated myotubes, and the increase of MHC-positive immunoreactivity within the myotubes, further reflected a complete differentiation and maturation of the contractile skeletal muscle cells induced by teaghrelin. Like ghrelin, teaghrelin attenuated dexamethasone-decreased myotube diameter, indicating its protective effects against skeletal muscle atrophy. Additionally, the expressions of Atrogin-1 and MuRF-1 ubiquitin E3 ligase were reduced. In conclusion, the results highlight a possibility of developing teaghrelin as a functional food for the prevention or therapeutic treatment of disease-associated skeletal muscle atrophy.

中文翻译：

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提格列林处理的小鼠C2C12成肌细胞中肌管分化的促进和肌肉萎缩的减弱。

这项研究旨在研究茶新素乌龙茶（钦新乌龙茶的有效成分）对小鼠C2C12成肌细胞的影响。在高血清条件下，teaghrelin抑制C2C12细胞增殖，表明细胞周期停滞和增殖进程停止。Teaghrelin促进C2C12细胞的前分化，这由逐渐延长的形态以及诱导肌肉特异性肌生成素，肌球蛋白重链（MHC）和MyoD所证明。多核肌管的形成，以及肌管内MHC阳性免疫反应性的增加，进一步反映了由茶黄素诱导的收缩性骨骼肌细胞的完全分化和成熟。像生长素释放肽一样，生长素释放肽可降低地塞米松降低的肌管直径，说明其对骨骼肌萎缩的保护作用。此外，Atrogin-1和MuRF-1泛素E3连接酶的表达降低。总之，这些结果突出了发展茶黄素作为预防或治疗与疾病相关的骨骼肌萎缩的功能性食品的可能性。