Methyltransferases (MTases) are enzymes that modify specific substrates by adding a methyl group using S-adenosyl-l-methionine. Functions of MTases have been extensively studied in eukaryotic organisms and animal pathogenic bacteria. Despite their importance, mechanisms underlying MTase function in plant pathogenic bacteria have not been studied in depth, as is the case of Xanthomonas axonopodis pv. glycines (Xag) that causes bacterial pustule disease in soybean crops worldwide. Here, the association between Xag proteome alterations and three MTase-overexpressing strains, Xag(XgMT1), Xag(XgMT2), and Xag(XgMT3), compared to Xag carrying an empty vector, Xag(EV) is reported. Using label-free shotgun comparative proteomic analysis, proteins are identified in all three biological replicates of the four strains and ranged from 1004 to 1082. In comparative analyses, 124, 135, and 134 proteins are differentially changed (over twofold) by overexpression of XgMT1, XgMT2, and XgMT3, respectively. These proteins are also categorized using cluster of orthologous group (COG) analyses, allowing postulation of biological mechanisms associated with three MTases in Xag. COGs reveal that the three MTases may play distinct roles, although some functions may overlap. These results are expected to allow new insight into understanding and predicting the biological functions of MTases in plant pathogenic bacteria. Data are available via ProteomeXchange (Identifier PXD012590).

中文翻译：

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通过 Xanthomonas axonopodis pv. 中三种假定的甲基转移酶的过表达来分析差异丰富的蛋白质。甘氨酸。


甲基转移酶 (MTase) 是通过使用 S-腺苷-L-甲硫氨酸添加甲基来修饰特定底物的酶。 MTase 的功能已在真核生物和动物病原菌中得到广泛研究。尽管它们很重要，但植物病原菌中 MTase 功能的机制尚未得到深入研究，就像 Xanthomonas axonopodis pv. 的情况一样。甘氨酸 (Xag) 在全世界大豆作物中引起细菌性脓疱病。在此，报告了 Xag 蛋白质组改变与三种 MTase 过表达菌株 Xag(XgMT1)、Xag(XgMT2) 和 Xag(XgMT3) 之间的关联，与携带空载体 Xag(EV) 的 Xag 相比。使用无标记鸟枪比较蛋白质组分析，在四个菌株的所有三个生物重复中鉴定出蛋白质，范围从 1004 到 1082。在比较分析中，124、135 和 134 个蛋白质因 XgMT1 的过度表达而发生差异性改变（超过两倍） 、 XgMT2 和 XgMT3 分别。这些蛋白质还使用直系同源群 (COG) 分析簇进行分类，从而可以推测与 Xag 中的三种 MTase 相关的生物机制。 COG 显示这三种 MTase 可能发挥不同的作用，尽管某些功能可能重叠。这些结果有望为理解和预测植物病原菌中 MTase 的生物学功能提供新的见解。数据可通过 ProteomeXchange 获取（标识符 PXD012590）。