Abstract Introduction: Clinical differential diagnosis of endometrial carcinoma is challenging, as signs and symptoms may vary considerably and there is a lack of reliable diagnostic serum biomarkers. Aim of the study: The aim of our work was to characterize the deoxyribonucleic acid and chromatin changes in the tissue of patients confirmed to be suffering from endometriosis and endometrial adenocarcinoma and compare it with a healthy control group. Material and methods: All samples were collected during recommended surgical interventions, and after the DNA isolation, a sonification followed by crosslink of chromatin were done. Consequently, both DNA and chromatin were examined using atomic force microscopy. Results: A chromatin immunoprecipitation was used for the in vivo observation of conformational chromatin changes. The width of ssDNA showed a significant difference, almost double the control value in the endometrial cancer sample versus control (by 73 ± 5% wider, p < 0.001). In contrast, the height of ssDNA was highest in the frozen pelvis patient sample (by 510 ± 12% compared to control, p < 0.01). Conclusion: Our results suggest that the horizontal size of single-stranded deoxyribonucleic acid and nucleosomes can help to identify potential patients with endometrial adenocarcinoma, while the height of the same parameter is associated with endometriosis.

中文翻译：

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使用原子力显微镜对子宫内膜异位症和子宫内膜癌进行脱氧核糖核酸和染色质成像

摘要介绍：子宫内膜癌的临床鉴别诊断具有挑战性，因为体征和症状可能差异很大，并且缺乏可靠的诊断血清生物标志物。研究目的：我们工作的目的是表征确诊患有子宫内膜异位症和子宫内膜腺癌的患者组织中的脱氧核糖核酸和染色质变化，并将其与健康对照组进行比较。材料和方法：所有样本均在推荐的手术干预期间收集，DNA 分离后，进行染色质交联处理。因此，使用原子力显微镜检查 DNA 和染色质。结果：染色质免疫沉淀用于体内观察染色质构象变化。ssDNA 的宽度显示出显着差异，子宫内膜癌样本中的对照值几乎是对照值的两倍（宽 73 ± 5%，p < 0.001）。相比之下，ssDNA 的高度在冷冻骨盆患者样本中最高（与对照相比高 510 ± 12%，p < 0.01）。结论：我们的结果表明，单链脱氧核糖核酸和核小体的水平大小有助于识别潜在的子宫内膜腺癌患者，而同一参数的高度与子宫内膜异位症有关。