A novel LEAP-2 in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) confers protection against bacteria-stimulated inflammatory response.,Comparative Biochemistry and Physiology C: Toxicology & Pharmacology

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A novel LEAP-2 in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) confers protection against bacteria-stimulated inflammatory response.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-11-07 , DOI: 10.1016/j.cbpc.2019.108665
Sheng-Wei Luo ₁ , Kai-Kun Luo ₁ , Shao-Jun Liu ₁

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LEAP-2, a multifunctional peptide, not only exhibits a regulatory role in pathogenic infection, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-LEAP-2 was 240 bp and encoded 79 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-LEAP-2 was observed in liver. Aeromonas hydrophila challenge can sharply increase WR-LEAP-2 mRNA expression in liver, kidney and spleen. The purified WR-LEAP-2 peptide can directly bind to A. hydrophila and S. agalactiae, reduce the relative bacterial activity and limit bacterial growth in vitro. In addition, the treatment of WR-LEAP-2 can restrict bacterial dissemination in vivo and reduce production of pro-inflammatory cytokines. These results indicated that WR-LEAP-2 can confer protection against A. hydrophila- or S. agalactiae-stimulated MyD88-dependent pro-inflammatory cytokines activation.

中文翻译：

二倍体杂种鱼（Car鱼×♀鱼红色变种）中的新型LEAP-2赋予了针对细菌刺激的炎症反应的保护作用。

LEAP-2是一种多功能肽，不仅在致病性感染中发挥调节作用，而且还参与硬骨鱼类免疫力的调节。在这项研究中，WR-LEAP-2的ORF序列为240 bp，编码79个氨基酸残基。组织特异性分析显示，在肝脏中观察到WR-LEAP-2的最高表达。嗜水气单胞菌攻击可急剧增加肝，肾和脾中WR-LEAP-2 mRNA的表达。纯化的WR-LEAP-2肽可以直接与亲水链球菌和无乳链球菌结合，降低相对细菌活性并限制细菌在体外的生长。另外，WR-LEAP-2的治疗可以限制细菌在体内的传播并减少促炎细胞因子的产生。这些结果表明WR-LEAP-2可以赋予针对亲水曲霉或S. S.的保护。

更新日期：2019-11-07

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