The reversible oxidation and reduction of Cytochrome c (Cyt c) is demonstrated with a closed bipolar electrochemical cell (CBPEC). Herein, a 4-electrode configuration was studied with the opposite poles of the bipolar electrode resting in separate aqueous and organic electrolyte solutions, respectively. Using biocompatible indium tin oxide (ITO) slides as the bipolar electrode poles, we investigated the influence of the redox potential of the reductant (decamethyferrocene or dimethylferrocene) in an α,α,α-trifluorotoluene organic phase on the observed voltammetry. Reversible electron transfer was only observed between Cyt c and decamethylferrocene. Use of the weaker dimethylferrocene as the reductant required a larger external bias of the driving electrodes to initiate the electron transfer reaction between the two poles of the bipolar electrode. Consequently, the surface of the ITO slide at the aqueous pole experienced a significant negative cathodic potential and underwent irreversible reduction. The biphasic setup using the 4-electrode CBPEC provides insights into electron transfer processes at an interface between two immiscible electrolyte solutions (ITIES), highlighting the strong probability of observing interfacial electron transfer between decamethylferrocene (but not dimethylferrocene) and Cyt c within the short ∼ 1 V polarisable potential window available at an ITIES.

用封闭的双极电化学池（CBPEC）证明了细胞色素c（Cyt c）的可逆氧化和还原。在此，研究了一种四电极配置，双极电极的相反两极分别放置在单独的水溶液和有机电解质溶液中。使用生物相容的铟锡氧化物（ITO）玻片作为双极电极极，我们研究了α，α，α-三氟甲苯有机相中还原剂（癸二茂铁或二甲基二茂铁）的氧化还原电势对观察到的伏安法的影响。仅在Cyt c之间观察到可逆的电子转移和十甲基二茂铁。使用较弱的二甲基二茂铁作为还原剂需要驱动电极更大的外部偏压以引发双极电极的两个极之间的电子转移反应。因此，ITO玻片在水极处的表面经历了显着的负阴极电势，并且发生了不可逆的还原。使用4电极CBPEC的双相​​设置可洞悉两种不混溶的电解质溶液（ITIES）之间的界面处的电子转移过程，从而突出了在很短的时间内观察到十甲基二茂铁（而非二甲基二茂铁）和Cyt c之间界面电子转移的强大可能性。ITY提供1 V极化电位窗口。