The link between Neuraminidase 1 (Neu1) and cancer development has been highlighted in numerous studies. In an effort to understand the role of Neu1 in mammary carcinoma cells, we evaluated the effect of Neu1 on controlling cell proliferation and apoptosis, as well as regulating the expression of cadherins. By blocking the activity of Neu1 with oseltamivir phosphate or using siRNA to silence the Neu1 protein, we observed suppression of cell growth in MCF-7 and MDA-MB-231 cells. Enhanced cleaved caspase 3 expression was demonstrated in breast cancer cells treated with oseltamivir phosphate or in Neu1 knockdown mammary carcinoma cells. We also provided evidence of Neu1 reversing the epithelial-mesenchymal properties with associated changes to the respective cadherin family. Additional observations indicated that the phytochemical, honokiol downregulates the expression of Neu1. As a consequence of blocking Neu1, honokiol reduced the levels of sialic acid in the two subtypes of breast cancer. These findings provide evidence that Neu1 regulates cell growth and death, and facilitates cancer progression by modulating the expression levels of cadherins.

中文翻译：

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神经氨酸酶 1 调节乳腺癌细胞中钙粘蛋白的增殖、凋亡和表达。

许多研究都强调了神经氨酸酶 1 (Neu1) 与癌症发展之间的联系。为了了解 Neu1 在乳腺癌细胞中的作用，我们评估了 Neu1 对控制细胞增殖和凋亡以及调节钙粘蛋白表达的作用。通过用磷酸奥司他韦阻断 Neu1 的活性或使用 siRNA 沉默 Neu1 蛋白，我们观察到 MCF-7 和 MDA-MB-231 细胞中的细胞生长受到抑制。在用磷酸奥司他韦处理的乳腺癌细胞或 Neu1 敲低的乳腺癌细胞中证实了增强的切割半胱天冬酶 3 表达。我们还提供了 Neu1 逆转上皮间充质特性的证据，并与相应的钙粘蛋白家族相关的变化。额外的观察表明，植物化学物质，和厚朴酚下调 Neu1 的表达。作为阻断 Neu1 的结果，和厚朴酚降低了两种乳腺癌亚型中的唾液酸水平。这些发现提供了证据表明 Neu1 调节细胞生长和死亡，并通过调节钙粘蛋白的表达水平促进癌症进展。