Thraustochytriidae sp., have drawn the attention as a promising source for producing significant quantities of omega‐3 fatty acids. In this study, Thraustochytriidae sp. PKU#Mn16, with impressive docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) production, was de novo assembled as a complete transcriptome. A total of 59.65 Mb clean reads were assembled into 35,745 transcripts, with a mean size of 991 bp. The results allowed us to annotate 11,924 (45.97%) of the transcripts with putative functions. The potential target genes enriched for specific gene ontology terms, including metabolic process (469 genes) and macromolecular complex (221 genes), were identified. There were 6858 (26.44%) putative proteins classified into 25 categories, of which 459 (6.69%) predicted proteins were assigned to lipid transport and metabolism, closely related to DHA production. The most strongly represented pathways annotated from the kyoto encyclopedia of genes and genomes (KEGG) database were metabolism pathways including carbohydrate (388, 6.99%), amino acid (375, 6.76%), and lipid (330, 5.95%). Of these unigenes with coding sequences, the majority (2950) were 200–300 bp. A total of 7362 simple sequence repeats were identified with the frequency occurrence of 1/4.15 kb. This study reveals a wealth of the most comprehensive transcriptomic resource currently available for Thraustochytriidae sp., which could facilitate further research into the molecular mechanisms of fatty acid production.

中文翻译：

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De Novo转录组分析海洋原生生物Thraustochytriidae sp。中的多不饱和脂肪酸代谢。北大＃Mn16

破囊壶菌科已经引起了人们的注意，因为它是产生大量omega-3脂肪酸的有前途的来源。在这项研究中，破囊壶菌科sp。重新生产了具有令人印象深刻的二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）的PKU＃Mn16。总共59.65 Mb干净读段被组装成35,745个转录本，平均大小为991 bp。结果使我们能够用推定功能注释11,924（45.97％）个成绩单。确定了富含特定基因本体术语的潜在靶基因，包括代谢过程（469个基因）和大分子复合物（221个基因）。推定的蛋白质有6858种（26.44％）分为25类，其中459种蛋白质（6.69％）预测的蛋白质被分配给脂质转运和代谢，与DHA产生密切相关。从京都百科全书的基因和基因组数据库（KEGG）中最有代表性的途径是代谢途径，包括碳水化合物（388，6.99％），氨基酸（375，6.76％）和脂质（330，5.95％）。在这些具有编码序列的单基因中，大多数（2950）为200–300 bp。总共鉴定到7362个简单序列重复，频率为1 / 4.15 kb。这项研究揭示了当前可用于大量的最全面的转录组学资源破囊壶菌科，其可以促进对脂肪酸产生的分子机理的进一步研究。