Upon storage, crude plant oils will form a solid sediment called gum, which consists mainly of phospholipids (PL). PL are removed during the production of edible oils by a process called degumming. A higher yield is recognized as a major advantage of enzymatic degumming over traditional processes. Robust and accurate PL quantification methods are needed to develop and monitor enzymatic degumming processes. Several techniques, such as atomic emission spectroscopy, liquid chromatography, and thin‐layer chromatography, have been applied for the quantification of PL in edible oils. In the past decade, ³¹P NMR spectroscopy has been shown to have advantages over these techniques because of the possibility of the simultaneous, fast, and accurate quantification of different PL directly in the oil. This article demonstrates the application of ³¹P NMR spectroscopy as a method for the quantification of all relevant PL and phosphorous‐containing degradation products in crude and refined oils. In addition, the validation results show that this method is robust because the limit of detection is as low as 5 μmol/100 g oil. Variations of less than 5% were obtained for all P‐containing compounds present in the oils at concentrations above 100 μmol/100 g oil.

中文翻译：

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使用31P NMR光谱对食用油中的磷脂进行可靠可靠的定量分析

储存后，粗植物油将形成称为树胶的固体沉积物，其主要由磷脂（PL）组成。PL在食用油生产过程中通过称为脱胶的过程除去。与传统方法相比，酶促脱胶的主要优点是产量更高。需要鲁棒和准确的PL定量方法来开发和监测酶脱胶过程。几种技术，例如原子发射光谱法，液相色谱法和薄层色谱法，已用于定量食用油中的PL。在过去的十年中，³¹由于直接，同时，快速，准确地定量油中不同PL的可能性，P NMR光谱已显示出优于这些技术的优势。本文展示了³¹ P NMR光谱作为定量分析原油和精炼油中所有相关PL和含磷降解产物的方法的应用。此外，验证结果表明该方法是可靠的，因为检测限低至5μmol/ 100 g油。当油中浓度高于100μmol/ 100 g油时，所有含P化合物的变化均小于5％。