The aim of the study was to evaluate the selected lymphocyte subpopulations TCD4, TCD8, BCD21, BCD25, CD18, CD11b, and MHC II in blood and uterine flush of cows with endometritis, before and after intrauterine (i.u.) administration of cefapirin and methisoprinol. The research was carried out on 28 cows with clinical endometritis. Animals were divided into four groups, each composed of seven cows, depending on the i.u. preparation used: Group A, cefapirin; Group B, methisoprinol; Group C, cefapirin and methisoprinol simultaneously; and a control group-without medication. The study was performed using flow cytometry method. Summarizing the results of the research, i.u. infusion of cefapirin caused a weakening of the effector phase of the local uterine immune response; however, it enhanced leukocyte chemotaxis and antigen presentation. After i.u. administration of methisoprinol, the stimulation of specific uterine immunity mechanisms was mainly observed. The use of both mentioned preparations showed the strengthening of specific uterine immunological mechanisms presumably caused by methisoprinol, despite the inhibitory effect of the antibiotic. Intrauterine use of immunostimulatory substances can improve the effectiveness of the endometritis treatment in cows by improving specific local mechanisms of uterine immunity. As a consequence, it may enhance the effector function of immune competent cells and finally eliminate inflammation.

中文翻译：

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在宫内施用头孢匹林和甲硫氧普林前后奶牛外周血和子宫清洗液中的选定白细胞亚群。

该研究的目的是评估在子宫内 (iu) 施用头孢匹林和甲硫索普林前后子宫内膜炎奶牛的血液和子宫冲洗液中选定的淋巴细胞亚群 TCD4、TCD8、BCD21、BCD25、CD18、CD11b 和 MHC II。该研究是在 28 头患有临床子宫内膜炎的奶牛身上进行的。根据所用的 iu 制剂，将动物分为四组，每组由七头牛组成：A 组，头孢匹林；A 组，头孢匹林。B组，甲氧萘普酚；C组，同时使用头孢匹林和甲硫索普林；和一个对照组——不服药。该研究使用流式细胞术方法进行。总结研究结果，iu 输注头孢匹林导致局部子宫免疫反应的效应期减弱；然而，它增强了白细胞趋化性和抗原呈递。在我之后 服用甲氧异丙醇，主要观察到特异性子宫免疫机制的刺激。尽管抗生素具有抑制作用，但上述两种制剂的使用均显示可能由甲硫氧萘酚引起的特定子宫免疫机制得到加强。宫内使用免疫刺激物质可以通过改善子宫免疫的特定局部机制来提高奶牛子宫内膜炎治疗的有效性。因此，它可以增强免疫活性细胞的效应功能，最终消除炎症。尽管有抗生素的抑制作用。宫内使用免疫刺激物质可以通过改善子宫免疫的特定局部机制来提高奶牛子宫内膜炎治疗的有效性。因此，它可以增强免疫活性细胞的效应功能，最终消除炎症。尽管有抗生素的抑制作用。宫内使用免疫刺激物质可以通过改善子宫免疫的特定局部机制来提高奶牛子宫内膜炎治疗的有效性。因此，它可以增强免疫活性细胞的效应功能，最终消除炎症。