An oxygen gradient formed along the length of colonic crypts supports stem-cell proliferation at the normoxic crypt base while supporting obligate anaerobe growth in the anoxic colonic lumen. Primary human colonic epithelial cells derived from human gastrointestinal stem cells were cultured within a device possessing materials of tailored oxygen permeability to produce an oxygen-depleted luminal (0.8% ± 0.1% O2) and oxygen-rich basal (11.1% ± 0.5% O2) compartment. This oxygen difference created a stable oxygen gradient across the colonic epithelial cells which remained viable and properly polarized. Facultative and obligate anaerobes Lactobacillus rhamnosus, Bifidobacterium adolescentis, and Clostridium difficile grew readily within the luminal compartment. When formed along the length of an in vitro crypt, the oxygen gradient facilitated cell compartmentalization within the crypt by enhancing confinement of the proliferative cells to the crypt base. This platform provides a simple system to create a physiological oxygen gradient across an intestinal mimic while simultaneously supporting anaerobe co-culture.

中文翻译：

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具有自持氧梯度的体外肠道平台，用于研究人肠/微生物组界面。

沿结肠隐窝长度形成的氧梯度支持常氧隐窝基部的干细胞增殖，同时支持缺氧结肠腔内专性厌氧菌的生长。在具有定制的氧气渗透性的材料的设备中培养源自人类胃肠干细胞的原代人结肠上皮细胞，以产生贫氧的腔（0.8％±0.1％O2）和富氧的基础气体（11.1％±0.5％O2）隔间。这种氧差异在整个结肠上皮细胞上产生了一个稳定的氧梯度，该梯度保持了活力并正确极化。兼性和专性厌氧菌鼠李糖乳杆菌，青春双歧杆菌和艰难梭菌在腔室内很容易生长。当沿着体外隐窝的长度形成时，氧梯度通过增强增殖细胞对隐窝碱基的限制而促进了隐窝内的细胞区室化。该平台提供了一个简单的系统，可在整个肠道模拟物中创建生理氧梯度，同时支持厌氧菌共培养。